840:153g:Projects/project23/2012/10/26

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Site-directed Mutagenesis

  • On Friday, we ran Thursday's PCR products on a 1.8% Agarose gel. The samples were loaded as follows:


  • Upper lane
  1. 100bp ladder
  2. Non-primered set without template
  3. Upstream fragment without template
  4. Primered set without template
  5. Downstream fragment without template
  6. 100 bp ladder
  7. Downstream fragment
  8. Primered set
  9. Upstream fragment
  10. Non-primered set


  • Lower lane
  1. Positive control
  2. 100 bp ladder
  • All other lanes are replications of primered set


  • Results:
  • Upper lane, wells 1-5
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