Casey Durnan: /* Entry title */

2012-11-09T02:48:14Z

Entry title

←Older revision		Revision as of 02:48, 9 November 2012
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-	* ~~Insert content here~~...	+	* Today, we photographed the gel from 11.01.2012. After photographing both gels, we cut out the bands from the 350bp and 450bp regions. We stored these gel slices at -20°C for further DNA extraction next week.
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		+	*1 percent agarose gel
		+	*1. 100-base pair ladder
		+	*2-4. Replicates of primered set
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		+	[[image:11.09.12_gel_1.0.jpg]]
		+	*Conclusion: These samples banded like all others before. We can use this for further extraction.
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		+	*1.8 percent agarose gel
		+	##Positive control
		+	##100 base-pair ladder
		+	##sfmr frag w/o DNA (neg control)
		+	##primered set w/o DNA (neg control)
		+	##mfsr frag w/o DNA (neg control)
		+	##100 base-pair ladder
		+	##sfmr frag w/ DNA
		+	##primered set w/ DNA
		+	##mfsr frag w/ DNA
		+	##100 base-pair ladder
		+	##primered replicate
		+	##primered replicate
		+	[[image:11.09.12_gel_1.8.jpg\|600px]]
		+	*Conclusion: Positive and negative controls check ok. For comparison, the primered set does have new, distinct banding from both fragments. After further calculations, our total product length should be ~350bp. A previous calculation did not take the overlap region between fragments into consideration. We will extract both the 350 and 450-bp regions to be certain. The replicates also look usable in this gel.
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	[[category:OWWLabNotebookV1]]		[[category:OWWLabNotebookV1]]

Casey Durnan: Autocreate 2012/11/02 Entry for 840:153g:Projects/project23

2012-11-09T02:28:38Z

Autocreate 2012/11/02 Entry for 840:153g:Projects/project23

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
* Insert content here...


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[[category:OWWLabNotebookV1]]

840:153g:Projects/project23/2012/11/02 - Revision history

Casey Durnan: /* Entry title */

Casey Durnan: Autocreate 2012/11/02 Entry for 840:153g:Projects/project23