840:153g:Projects/project23/2012/11/08: Difference between revisions
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== | ==Transforming E. coli with omcf gene== | ||
* On Tuesday, we extracted our DNA from gels stored 11.02.12. We used a gel-extraction kit and extracted both the 350- and 450-bp regions in 100 mcl of water each. | * On Tuesday, we extracted our DNA from gels stored 11.02.12. We used a gel-extraction kit and extracted both the 350- and 450-bp regions in 100 mcl of water each. | ||
* On Thursday, we digested the DNA samples from 11.08.12 using restriction enzymes PstI and XbaI for our Biobrick assembly. We then purified the reaction with a purification kit to remove restriction enzymes and stored the product at -20°C for further ligation next week. | * On Thursday, we digested the DNA samples from 11.08.12 using restriction enzymes PstI and XbaI for our Biobrick assembly. We then purified the reaction with a purification kit to remove restriction enzymes and stored the product at -20°C for further ligation next week. | ||
Revision as of 20:04, 8 November 2012
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Transforming E. coli with omcf gene
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