840:153g:Projects/project23/2012/11/15: Difference between revisions
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#2x plasmid DNA | #2x plasmid DNA | ||
#2x plasmid DNA | #2x plasmid DNA | ||
# | #4μL Low Range Ladder | ||
# | #2μL Low Range Ladder | ||
#450bp 2x DNA | #450bp 2x DNA | ||
#450bp 1x DNA | #450bp 1x DNA | ||
Line 21: | Line 21: | ||
#100bp Ladder | #100bp Ladder | ||
* We then ligated the samples with concentrations of 4:4, 0.5:7.5, and 7.5:0. | * We then ligated the samples with concentrations of 4:4, 0.5:7.5, and 7.5:0.5μL of plasmid DNA to our insert DNA (OmcF DNA). We also did 2 controls for each 450 and 350 DNA sample. One with no plasmid DNA and one with no insert DNA | ||
* On Thursday, we continued to work on the transformation, by actually combining our ligated samples with | * On Thursday, we continued to work on the transformation, by actually combining our ligated samples with 40μL of E.coli cells, icing, heat shocking, then icing the samples. We then added SOC and shook the samples for an hour at 37°C. | ||
* We then plated our samples on solid media plates. | * We then plated our samples on solid media plates. | ||
Revision as of 14:38, 15 November 2012
Cloning of the OmcF gene | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Cloning E.coli with the OmcF gene
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