840:153g:Projects/project23/2012/11/15: Difference between revisions
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#350bp 1x DNA | #350bp 1x DNA | ||
#100bp Ladder | #100bp Ladder | ||
[[image:Gel 11.13.12.jpg|600px]] | |||
*Result: Wells are shown No. 10 - 1 above. Plasmid DNA is intact, and our fragment DNAs are in correct position. Well 9 is overrepresented slightly, and all other sample combinations are comparable. The samples are useable for transformation, but in differing ratios to find the most effective combination. | |||
* We then ligated the samples with concentrations of 4:4, 0.5:7.5, and 7.5:0.5μL of plasmid DNA to our insert DNA (OmcF DNA). We also did 2 controls for each 450 and 350 DNA sample. One with no plasmid DNA and one with no insert DNA | * We then ligated the samples with concentrations of 4:4, 0.5:7.5, and 7.5:0.5μL of plasmid DNA to our insert DNA (OmcF DNA). We also did 2 controls for each 450 and 350 DNA sample. One with no plasmid DNA and one with no insert DNA |
Revision as of 12:18, 29 November 2012
Cloning of the OmcF gene | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Cloning E.coli with the OmcF gene
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