840:153g:Projects/project24

Team Members

• Name of student Sanju Timilsina
• Name of student Parul Sirohi

Over expression of diacyl- glycerol acyl transferase (DGAT) in Chlamydomonas reinhardtii to enhance tri-acyl glycerol production, the precursor of Bio-fuel production.

• Gene used: Diacyl glycerol acyl transferase (DGTA) gene

 Function of gene: DGTA is a precursor enzyme which regulates the final step bio-synthetic step of Triacyl glycerol production ( fatty acid which is used to produce bio-fuel).

Introduction: Transgenic microalgae has been the promising organism to study for biofuel production. Due to its ability to grow fast and product lipid, microalgae is the choice of organism for us. Specially in microalgae we are interested to see if the over expression of DGTA gene will enhance Triacyl glycerol production (TAG). TAG is the final by-product of fatty acid synthesis in micro algae. And DGTA is the regulatory enzyme for TAG bio-synthesis. The global synthesis pathway of TAG synthesis in cells ic comprised of three major steps: 1. Carboxylation of acetyl- CoA to form malonyl CoA 2.Acyl chain elongation 3.TAG formation.

• List all the steps that are needed to complete your project
• Do not go into experimental details and don't list procedures. Just list the major steps necessary to complete your project
• Please also make yourself familiar with uploading pictures and *.ppt files

Important Results and Milestones

• keep track of your most important results and refer to the corresponding page in your notebook
• upload important pictures (don't forget to label them! Powerpoint is very convenient). Remember: these will become quite handy later in your summary report or final presentation. If you do label and upload the pictures as soon as you got them, your summary report can be written much more effortlessly (do you usually procrastinate? This is chance to do some work before hand that frees you up for finals week).

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