840:153g:Projects/project24/2012/09/17
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Project title: Bio-fuel production by using genetically engineered microorganisms. | Project title: Bio-fuel production by using genetically engineered microorganisms. | ||
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| + | Source organism E coli culture: | ||
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| + | 1. 50ml of LB broth stored in -4 0C was poured in two conical flask . 50 ml each under aseptic condition( Bunsen burner on) | ||
| + | 2. Flask was then labelled 1 and 2, LB, date and group number . | ||
| + | 3.25ul E coli strain from -80 0C brought into room temperature was inoculated into flask 2 and 28ul into flask 1. | ||
| + | 4.Both flask were incubated into shaker, set at 37 0C, pressure 222for 28 hours. | ||
Revision as of 22:51, 24 September 2012
Team Members: Sanju Timilsina and Parul Sirohi
Project title: Bio-fuel production by using genetically engineered microorganisms.
Source organism E coli culture:
1. 50ml of LB broth stored in -4 0C was poured in two conical flask . 50 ml each under aseptic condition( Bunsen burner on) 2. Flask was then labelled 1 and 2, LB, date and group number . 3.25ul E coli strain from -80 0C brought into room temperature was inoculated into flask 2 and 28ul into flask 1. 4.Both flask were incubated into shaker, set at 37 0C, pressure 222for 28 hours.
