840:153g:Projects/project24/2012/09/17

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Source organism E coli culture:
Source organism E coli culture:
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1. 50ml of LB broth stored in -4 0C was poured in two conical flask . 50 ml each under aseptic condition( Bunsen burner on)
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# 50ml of LB broth stored in -4 0C was poured in two conical flask . 50 ml each under aseptic condition( Bunsen burner on)
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2. Flask was then labelled 1 and 2, LB, date and group number .
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# Flask was then labelled 1 and 2, LB, date and group number .
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3.25ul E coli strain from -80 0C brought into room temperature was inoculated into flask 2 and 28ul into flask 1.
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#25ul E coli strain from -80 0C brought into room temperature was inoculated into flask 2 and 28ul into flask 1.
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4.Both flask were incubated into shaker, set at 37 0C, pressure 222for 28 hours.
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#Both flask were incubated into shaker, set at 37 0C, pressure 222for 28 hours.

Revision as of 22:54, 24 September 2012

Team Members: Sanju Timilsina and Parul Sirohi

Project title: Bio-fuel production by using genetically engineered microorganisms.

Source organism E coli culture:

  1. 50ml of LB broth stored in -4 0C was poured in two conical flask . 50 ml each under aseptic condition( Bunsen burner on)
  2. Flask was then labelled 1 and 2, LB, date and group number .
  3. 25ul E coli strain from -80 0C brought into room temperature was inoculated into flask 2 and 28ul into flask 1.
  4. Both flask were incubated into shaker, set at 37 0C, pressure 222for 28 hours.
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