840:153g:Projects/project24/2012/09/17: Difference between revisions
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# Flask was then labelled 1 and 2, LB, date and group number . | # Flask was then labelled 1 and 2, LB, date and group number . | ||
#25ul E coli strain from -80 0C brought into room temperature was inoculated into flask 2 and 28ul into flask 1. | #25ul E coli strain from -80 0C brought into room temperature was inoculated into flask 2 and 28ul into flask 1. | ||
#Both flask were incubated into shaker, set at 37 0C, pressure 222for 28 hours. | #Both flask were incubated into shaker, set at 37 0C, pressure 222for 28 hours and we decided to take our sample out of the shaker before our next day class. |
Revision as of 21:15, 25 September 2012
Team Members: Sanju Timilsina and Parul Sirohi
Project title: Bio-fuel production by using genetically engineered microorganisms.
Source organism E coli culture:
- 50ml of LB broth stored in -4 0C was poured in two conical flask . 50 ml each under aseptic condition( Bunsen burner on)
- Flask was then labelled 1 and 2, LB, date and group number .
- 25ul E coli strain from -80 0C brought into room temperature was inoculated into flask 2 and 28ul into flask 1.
- Both flask were incubated into shaker, set at 37 0C, pressure 222for 28 hours and we decided to take our sample out of the shaker before our next day class.