840:153g:Projects/project24/2012/09/17

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Line 8: Line 8:
# Flask was then labelled 1 and 2, LB, date and group number .
# Flask was then labelled 1 and 2, LB, date and group number .
#25ul E coli strain from -80 0C brought into room temperature was inoculated into flask 2 and 28ul into flask 1.
#25ul E coli strain from -80 0C brought into room temperature was inoculated into flask 2 and 28ul into flask 1.
-
#Both flask were incubated into shaker, set at 37 0C, pressure 222for 28 hours.
+
#Both flask were incubated into shaker, set at 37 0C, pressure 222for 28 hours and we decided to take our sample out of the shaker before our next day class.

Revision as of 23:15, 25 September 2012

Team Members: Sanju Timilsina and Parul Sirohi

Project title: Bio-fuel production by using genetically engineered microorganisms.

Source organism E coli culture:

  1. 50ml of LB broth stored in -4 0C was poured in two conical flask . 50 ml each under aseptic condition( Bunsen burner on)
  2. Flask was then labelled 1 and 2, LB, date and group number .
  3. 25ul E coli strain from -80 0C brought into room temperature was inoculated into flask 2 and 28ul into flask 1.
  4. Both flask were incubated into shaker, set at 37 0C, pressure 222for 28 hours and we decided to take our sample out of the shaker before our next day class.
Personal tools