840:153g:Projects/project24/2012/10/18 - Revision history

PARUL Sirohi: /* Title here */

PARUL Sirohi — Thu, 29 Nov 2012 20:56:12 GMT

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-	On Tuesday, We did agarose gel electrophoresis for PCR samples to check for amplification ~~RDT_gel_pictures.tif‎ (TIFF file, nominally 960 × 720 pixels, file size: 225 KB)~~. we did not get any amplification in DNA samples however, we got the positive control band. We made an interpretation from the gel that may be primers did not bind at their specific sites or may be there is some problem in DNA sample. Since we are running out of our DNA sample, we decided to do the DNA extraction.This time we will be comparing two different protocols on parallel. One that we used before i.e, DNA extraction for	+	On Tuesday, We did agarose gel electrophoresis for PCR samples to check for amplification. we did not get any amplification in DNA samples however, we got the positive control band. We made an interpretation from the gel that may be primers did not bind at their specific sites or may be there is some problem in DNA sample. Since we are running out of our DNA sample, we decided to do the DNA extraction.This time we will be comparing two different protocols on parallel. One that we used before i.e, DNA extraction for
	E.coli chromosome and other that group 25 used which is, Bacterial Genomic DNA extraction protocol.		E.coli chromosome and other that group 25 used which is, Bacterial Genomic DNA extraction protocol.

Sanju Timilsina: /* Title here */

Sanju Timilsina — Tue, 23 Oct 2012 14:17:13 GMT

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-	On Tuesday, We did agarose gel electrophoresis for PCR samples to check for amplification ~~10:08~~, ~~23 October 2012~~. we did not get any amplification in DNA samples however, we got the positive control band. We made an interpretation from the gel that may be primers did not bind at their specific sites or may be there is some problem in DNA sample. Since we are running out of our DNA sample, we decided to do the DNA extraction.This time we will be comparing two different protocols on parallel. One that we used before i.e, DNA extraction for	+	On Tuesday, We did agarose gel electrophoresis for PCR samples to check for amplification RDT_gel_pictures.tif‎ (TIFF file, nominally 960 × 720 pixels, file size: 225 KB). we did not get any amplification in DNA samples however, we got the positive control band. We made an interpretation from the gel that may be primers did not bind at their specific sites or may be there is some problem in DNA sample. Since we are running out of our DNA sample, we decided to do the DNA extraction.This time we will be comparing two different protocols on parallel. One that we used before i.e, DNA extraction for
	E.coli chromosome and other that group 25 used which is, Bacterial Genomic DNA extraction protocol.		E.coli chromosome and other that group 25 used which is, Bacterial Genomic DNA extraction protocol.

Sanju Timilsina: /* Title here */

Sanju Timilsina — Tue, 23 Oct 2012 14:11:34 GMT

Title here

←Older revision		Revision as of 14:11, 23 October 2012
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-	On Tuesday, We did agarose gel electrophoresis for PCR samples to check for amplification ~~http~~:~~//openwetware.org/wiki/Image:%21OCT15-P.CR.tif~~. we did not get any amplification in DNA samples however, we got the positive control band. We made an interpretation from the gel that may be primers did not bind at their specific sites or may be there is some problem in DNA sample. Since we are running out of our DNA sample, we decided to do the DNA extraction.This time we will be comparing two different protocols on parallel. One that we used before i.e, DNA extraction for	+	On Tuesday, We did agarose gel electrophoresis for PCR samples to check for amplification 10:08, 23 October 2012. we did not get any amplification in DNA samples however, we got the positive control band. We made an interpretation from the gel that may be primers did not bind at their specific sites or may be there is some problem in DNA sample. Since we are running out of our DNA sample, we decided to do the DNA extraction.This time we will be comparing two different protocols on parallel. One that we used before i.e, DNA extraction for
	E.coli chromosome and other that group 25 used which is, Bacterial Genomic DNA extraction protocol.		E.coli chromosome and other that group 25 used which is, Bacterial Genomic DNA extraction protocol.

PARUL Sirohi: /* Title here */

PARUL Sirohi — Mon, 22 Oct 2012 19:49:17 GMT

Title here

←Older revision		Revision as of 19:49, 22 October 2012
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	[[category:OWWLabNotebookV1]]		[[category:OWWLabNotebookV1]]

-	On Tuesday, We did agarose gel electrophoresis for PCR samples to check for amplification. we did not get any amplification in DNA samples however, we got the positive control band. We made an interpretation from the gel that may be primers did not bind at their specific sites or may be there is some problem in DNA sample. Since we are running out of our DNA sample, we decided to do the DNA extraction.This time we will be comparing two different protocols on parallel. One that we used before i.e, DNA extraction for	+	On Tuesday, We did agarose gel electrophoresis for PCR samples to check for amplification http://openwetware.org/wiki/Image:%21OCT15-P.CR.tif. we did not get any amplification in DNA samples however, we got the positive control band. We made an interpretation from the gel that may be primers did not bind at their specific sites or may be there is some problem in DNA sample. Since we are running out of our DNA sample, we decided to do the DNA extraction.This time we will be comparing two different protocols on parallel. One that we used before i.e, DNA extraction for
	E.coli chromosome and other that group 25 used which is, Bacterial Genomic DNA extraction protocol.		E.coli chromosome and other that group 25 used which is, Bacterial Genomic DNA extraction protocol.

PARUL Sirohi at 21:40, 18 October 2012

PARUL Sirohi — Thu, 18 Oct 2012 21:40:43 GMT

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		+
		+	On Tuesday, We did agarose gel electrophoresis for PCR samples to check for amplification. we did not get any amplification in DNA samples however, we got the positive control band. We made an interpretation from the gel that may be primers did not bind at their specific sites or may be there is some problem in DNA sample. Since we are running out of our DNA sample, we decided to do the DNA extraction.This time we will be comparing two different protocols on parallel. One that we used before i.e, DNA extraction for
		+	E.coli chromosome and other that group 25 used which is, Bacterial Genomic DNA extraction protocol.
		+
		+	On Wednesday, we did the culture to grow the E. coli. cells in four flasks and incubated in shaker for overnight.
		+
		+	On Thursday, since we did not have enough killing buffer we did the extraction with the genomic DNA extraction protocol only. We put rest of the three culture flasks at 4ºC.We will do agarose gel electrophoresis for these DNA samples in our next lab.

PARUL Sirohi: Autocreate 2012/10/18 Entry for 840:153g:Projects/project24

PARUL Sirohi — Thu, 18 Oct 2012 21:06:31 GMT

Autocreate 2012/10/18 Entry for 840:153g:Projects/project24

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{|{{table}} width="800"
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project Name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|-
| colspan="2"|

==Title here==


|}

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