840:153g:Projects/project25 - Revision history

http://www.openwetware.org/index.php?title=840:153g:Projects/project25&feed=atom&action=history 840:153g:Projects/project25 - Revision history 2013-05-22T08:54:42Z Revision history for this page on the wiki MediaWiki 1.13.2 http://www.openwetware.org/index.php?title=840:153g:Projects/project25&diff=662339&oldid=prev Bess E. Lippmann at 01:42, 5 December 2012 2012-12-05T01:42:40Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 01:42, 5 December 2012</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 54:</td> <td colspan="2" class="diff-lineno">Line 54:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Results & Conclusions ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Results & Conclusions ==</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>''To see our full results and conclusions presentation:'' [[http://openwetware.org/wiki/Image:<del class="diffchange diffchange-inline">25_Recombinant_Presentation</del>.pdf]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>''To see our full results and conclusions presentation:'' [[http://openwetware.org/wiki/Image:<ins class="diffchange diffchange-inline">FINAL_RecombPPT</ins>.pdf]]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==References==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==References==</div></td></tr>  </table>

Bess E. Lippmann http://www.openwetware.org/index.php?title=840:153g:Projects/project25&diff=662337&oldid=prev Bess E. Lippmann at 01:39, 5 December 2012 2012-12-05T01:39:13Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 01:39, 5 December 2012</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 52:</td> <td colspan="2" class="diff-lineno">Line 52:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Grow E. coli bacteria on experimental plates (No ampicillin, ampicillin, and ampicillin + L-arabinose)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Grow E. coli bacteria on experimental plates (No ampicillin, ampicillin, and ampicillin + L-arabinose)</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Verification testing by examining the physical cell shapes under a light microscope and sequence verification by sending a sample of cloned DNA to Iowa State.  </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Verification testing by examining the physical cell shapes under a light microscope and sequence verification by sending a sample of cloned DNA to Iowa State.  </div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">== Results & Conclusions ==</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">''To see our full results and conclusions presentation:'' [[http://openwetware.org/wiki/Image:25_Recombinant_Presentation.pdf]]</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==References==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==References==</div></td></tr>  </table>

Bess E. Lippmann http://www.openwetware.org/index.php?title=840:153g:Projects/project25&diff=631457&oldid=prev Bess E. Lippmann at 15:45, 2 October 2012 2012-10-02T15:45:07Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 15:45, 2 October 2012</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 36:</td> <td colspan="2" class="diff-lineno">Line 36:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* '''Primers:''' ''Forward primer:'' the first 34 bases of our gene sequence. We also added in the Biobrick enzymes EcoR1 and Xba1 in front of the primer. ''Reverse primer:'' the last 20 bases of our gene sequence. We also added in the Biobrick enzymes Pst1 and Spe1 in front of the primer. [[http://openwetware.org/wiki/Image:Primer_Picture1.png]], [[http://openwetware.org/wiki/Image:Primer_Pic2.png]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* '''Primers:''' ''Forward primer:'' the first 34 bases of our gene sequence. We also added in the Biobrick enzymes EcoR1 and Xba1 in front of the primer. ''Reverse primer:'' the last 20 bases of our gene sequence. We also added in the Biobrick enzymes Pst1 and Spe1 in front of the primer. [[http://openwetware.org/wiki/Image:Primer_Picture1.png]], [[http://openwetware.org/wiki/Image:Primer_Pic2.png]]</div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Forward Primer: 5' ATGTTCTCTTCCCTTTTCGGCGTGATCTCGAACG 3'                                                                              </ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Reverse Primer: 5' CTAGGCCAGCGTGGATTCCA 3'</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* '''Promoter:''' BBa_K206000 (pBad Strong Promoter). This promoter is inducible by L-arabinose. When induced, AraC binds and changes the conformation interacting with Aral1 and Aral2 operator sites, permitting transcription.  </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* '''Promoter:''' BBa_K206000 (pBad Strong Promoter). This promoter is inducible by L-arabinose. When induced, AraC binds and changes the conformation interacting with Aral1 and Aral2 operator sites, permitting transcription.  </div></td></tr>  </table>

Bess E. Lippmann http://www.openwetware.org/index.php?title=840:153g:Projects/project25&diff=625220&oldid=prev Bess E. Lippmann at 21:04, 11 September 2012 2012-09-11T21:04:15Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 21:04, 11 September 2012</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 42:</td> <td colspan="2" class="diff-lineno">Line 42:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Procedure ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Procedure ==</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* Grow ''C. crescentus'' on<del class="diffchange diffchange-inline">..</del>media and ''E. coli'' on LB media</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* Grow ''C. crescentus'' on <ins class="diffchange diffchange-inline">PYE </ins>media and ''E. coli'' on LB media</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* PCR to amplify mreB gene + Biobrick (BB) genes  </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* PCR to amplify mreB gene + Biobrick (BB) genes  </div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Isolate and purify mreB + BB genes  </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Isolate and purify mreB + BB genes  </div></td></tr>   </table>

Bess E. Lippmann http://www.openwetware.org/index.php?title=840:153g:Projects/project25&diff=625219&oldid=prev Bess E. Lippmann at 20:57, 11 September 2012 2012-09-11T20:57:23Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 20:57, 11 September 2012</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 17:</td> <td colspan="2" class="diff-lineno">Line 17:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Colby Swanson  </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Colby Swanson  </div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>==mreB <del class="diffchange diffchange-inline">Project Description</del>==</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>==<ins class="diffchange diffchange-inline">Cloning of </ins>mreB <ins class="diffchange diffchange-inline">from ''Caulobacter crescentus'' into ''E. coli''</ins>==</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''To see our full project description see initial project presentation:'' [[http://openwetware.org/wiki/Image:25_Recombinant_Presentation.pdf]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''To see our full project description see initial project presentation:'' [[http://openwetware.org/wiki/Image:25_Recombinant_Presentation.pdf]]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td colspan="2" class="diff-lineno">Line 42:</td> <td colspan="2" class="diff-lineno">Line 42:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Procedure ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Procedure ==</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* Grow ''C. crescentus'' and ''E. coli'' on <del class="diffchange diffchange-inline">the respective medias</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* Grow ''C. crescentus'' <ins class="diffchange diffchange-inline">on..media </ins>and ''E. coli'' on <ins class="diffchange diffchange-inline">LB media</ins></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* PCR to <del class="diffchange diffchange-inline">extract </del>mreB gene + Biobrick (BB) <del class="diffchange diffchange-inline">enzymes </del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* PCR to <ins class="diffchange diffchange-inline">amplify </ins>mreB gene + Biobrick (BB) <ins class="diffchange diffchange-inline">genes </ins></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* Isolate and purify mreB + BB <del class="diffchange diffchange-inline">enzymes </del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* Isolate and purify mreB + BB <ins class="diffchange diffchange-inline">genes </ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Cut mreB + BB enzymes and ligate with pSB1A3  </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Cut mreB + BB enzymes and ligate with pSB1A3  </div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Perform bacterial transformation to import the plasmid into ''E. coli''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Perform bacterial transformation to import the plasmid into ''E. coli''</div></td></tr>   </table>

Bess E. Lippmann http://www.openwetware.org/index.php?title=840:153g:Projects/project25&diff=625218&oldid=prev Bess E. Lippmann at 20:46, 11 September 2012 2012-09-11T20:46:56Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 20:46, 11 September 2012</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 49:</td> <td colspan="2" class="diff-lineno">Line 49:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Grow E. coli bacteria on experimental plates (No ampicillin, ampicillin, and ampicillin + L-arabinose)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Grow E. coli bacteria on experimental plates (No ampicillin, ampicillin, and ampicillin + L-arabinose)</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Verification testing by examining the physical cell shapes under a light microscope and sequence verification by sending a sample of cloned DNA to Iowa State.  </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Verification testing by examining the physical cell shapes under a light microscope and sequence verification by sending a sample of cloned DNA to Iowa State.  </div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==References==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==References==</div></td></tr>   </table>

Bess E. Lippmann http://www.openwetware.org/index.php?title=840:153g:Projects/project25&diff=625217&oldid=prev Bess E. Lippmann at 20:46, 11 September 2012 2012-09-11T20:46:00Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 20:46, 11 September 2012</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 35:</td> <td colspan="2" class="diff-lineno">Line 35:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* '''Gene of Interest:''' mreB from ''C. crescentus'' {Accession# NC_011916, 1,044 bp, no introns} [http://www.ncbi.nlm.nih.gov/nuccore/NC_011916.1?report=genbank&from=1733317&to=1734360]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* '''Gene of Interest:''' mreB from ''C. crescentus'' {Accession# NC_011916, 1,044 bp, no introns} [http://www.ncbi.nlm.nih.gov/nuccore/NC_011916.1?report=genbank&from=1733317&to=1734360]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* '''Primers:''' ''Forward primer:'' the first 34 bases of our gene sequence. We also added in the Biobrick enzymes EcoR1 and Xba1 in front of the primer. ''Reverse primer:'' the last 20 bases of our gene sequence. We also added in the Biobrick enzymes Pst1 and Spe1 in front of the primer.  </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* '''Primers:''' ''Forward primer:'' the first 34 bases of our gene sequence. We also added in the Biobrick enzymes EcoR1 and Xba1 in front of the primer. ''Reverse primer:'' the last 20 bases of our gene sequence. We also added in the Biobrick enzymes Pst1 and Spe1 in front of the primer. <ins class="diffchange diffchange-inline">[[http://openwetware.org/wiki/Image:Primer_Picture1.png]], [[http://openwetware.org/wiki/Image:Primer_Pic2.png]]</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* '''Promoter:''' BBa_K206000 (pBad Strong Promoter). This promoter is inducible by L-arabinose. When induced, AraC binds and changes the conformation interacting with Aral1 and Aral2 operator sites, permitting transcription.  </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* '''Promoter:''' BBa_K206000 (pBad Strong Promoter). This promoter is inducible by L-arabinose. When induced, AraC binds and changes the conformation interacting with Aral1 and Aral2 operator sites, permitting transcription.  </div></td></tr> <tr><td colspan="2" class="diff-lineno">Line 52:</td> <td colspan="2" class="diff-lineno">Line 52:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==References==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==References==</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Gitai, Z., & Yakhnina, A.A. (2012). The small protein mbiA interacts with mreB and modulates cell shape in ''caulobacter crescentus. Molecular Microbiology.'' doi: 10.1111/j.1365-2958.2012.08159.x</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Gitai, Z., & Yakhnina, A.A. (2012). The small protein mbiA interacts with mreB and modulates cell shape in ''caulobacter crescentus. Molecular Microbiology.'' doi: 10.1111/j.1365-2958.2012.08159.x <ins class="diffchange diffchange-inline">[http://www.ncbi.nlm.nih.gov/pubmed/22804814]</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Jacobs-Wagner, C., et al. (2012). Osmolality dependent relocation of penicillin-binding protein PBP2 to the division site in ''caulobacter crescentus. Journal of Bacteriology''. doi: 10.1128</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Jacobs-Wagner, C., et al. (2012). Osmolality dependent relocation of penicillin-binding protein PBP2 to the division site in ''caulobacter crescentus. Journal of Bacteriology''. doi: 10.1128 <ins class="diffchange diffchange-inline">[http://jb.asm.org/content/early/2012/04/11/JB.00260-12]</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>   </table>

Bess E. Lippmann http://www.openwetware.org/index.php?title=840:153g:Projects/project25&diff=625214&oldid=prev Bess E. Lippmann at 20:39, 11 September 2012 2012-09-11T20:39:14Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 20:39, 11 September 2012</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 18:</td> <td colspan="2" class="diff-lineno">Line 18:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==mreB Project Description==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==mreB Project Description==</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>To see our full project description see initial project presentation: [[http://openwetware.org/wiki/Image:25_Recombinant_Presentation.pdf]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">''</ins>To see our full project description see initial project presentation:<ins class="diffchange diffchange-inline">'' </ins>[[http://openwetware.org/wiki/Image:25_Recombinant_Presentation.pdf]]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The goal of our project is to clone the gene, mreB, from ''Caulobacter crescentus'' (stalked shaped cell) into ''Escherchia coli'' (rod shaped cell) to see if mreB will affect ''E. coli's'' cell shape in some way. We expect to see the ''E. coli'' cells lyse or change from their normal rod shape.  </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The goal of our project is to clone the gene, mreB, from ''Caulobacter crescentus'' (stalked shaped cell) into ''Escherchia coli'' (rod shaped cell) to see if mreB will affect ''E. coli's'' cell shape in some way. We expect to see the ''E. coli'' cells lyse or change from their normal rod shape.  </div></td></tr> <tr><td colspan="2" class="diff-lineno">Line 33:</td> <td colspan="2" class="diff-lineno">Line 33:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* '''Tissue Source:''' wild type ''C. crescentus'' CB15N (generously donated from the lab of Christine Jacobs-Wagner at Yale University)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* '''Tissue Source:''' wild type ''C. crescentus'' CB15N (generously donated from the lab of Christine Jacobs-Wagner at Yale University)</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* '''Gene of Interest:''' mreB from ''C. crescentus'' {Accession# NC_011916, 1,044 bp, no introns}</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* '''Gene of Interest:''' mreB from ''C. crescentus'' {Accession# NC_011916, 1,044 bp, no introns} <ins class="diffchange diffchange-inline">[http://www.ncbi.nlm.nih.gov/nuccore/NC_011916.1?report=genbank&from=1733317&to=1734360]</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* '''Primers:''' ''Forward primer:'' the first 34 bases of our gene sequence. We also added in the Biobrick enzymes EcoR1 and Xba1 in front of the primer. ''Reverse primer:'' the last 20 bases of our gene sequence. We also added in the Biobrick enzymes Pst1 and Spe1 in front of the primer.  </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* '''Primers:''' ''Forward primer:'' the first 34 bases of our gene sequence. We also added in the Biobrick enzymes EcoR1 and Xba1 in front of the primer. ''Reverse primer:'' the last 20 bases of our gene sequence. We also added in the Biobrick enzymes Pst1 and Spe1 in front of the primer.  </div></td></tr>   </table>

Bess E. Lippmann http://www.openwetware.org/index.php?title=840:153g:Projects/project25&diff=625213&oldid=prev Bess E. Lippmann at 20:36, 11 September 2012 2012-09-11T20:36:53Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 20:36, 11 September 2012</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 23:</td> <td colspan="2" class="diff-lineno">Line 23:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Normal ''E. coli'' cell shape [[http://openwetware.org/wiki/Image:E._coli_normal_cells]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Normal ''E. coli'' cell shape [[http://openwetware.org/wiki/Image:E._coli_normal_cells]]</div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Normal ''C. crescentus'' cell shape [[http://openwetware.org/wiki/Image:C._crescentus.jpg]]</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Lysed ''E. coli'' cells [[http://openwetware.org/wiki/Image:E._coli_cell_lysis.jpg]]</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Abnormal ''E. coli'' cells [[http://openwetware.org/wiki/Image:Morphed_e._coli_cells.gif]], [[http://openwetware.org/wiki/Image:E.Coli_MreB.jpg]]</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>MreB is a rod-shape determining gene that appears as bands or spirals encircling the cell. It is known to associate with mreC and penicillin-binding proteins to catalyze precursors for the peptidoglycan cell wall and correctly position polar bacterial proteins. It is also homologous of actin in eukaryotes.   </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>MreB is a rod-shape determining gene that appears as bands or spirals encircling the cell. It is known to associate with mreC and penicillin-binding proteins to catalyze precursors for the peptidoglycan cell wall and correctly position polar bacterial proteins. It is also homologous of actin in eukaryotes.   </div></td></tr>   </table>

Bess E. Lippmann http://www.openwetware.org/index.php?title=840:153g:Projects/project25&diff=625208&oldid=prev Bess E. Lippmann at 20:30, 11 September 2012 2012-09-11T20:30:14Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 20:30, 11 September 2012</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 18:</td> <td colspan="2" class="diff-lineno">Line 18:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==mreB Project Description==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==mreB Project Description==</div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">To see our full project description see initial project presentation: [[http://openwetware.org/wiki/Image:25_Recombinant_Presentation.pdf]]</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The goal of our project is to clone the gene, mreB, from ''Caulobacter crescentus'' (stalked shaped cell) into ''Escherchia coli'' (rod shaped cell) to see if mreB will affect ''E. coli's'' cell shape in some way. We expect to see the ''E. coli'' cells lyse or change from their normal rod shape.  </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The goal of our project is to clone the gene, mreB, from ''Caulobacter crescentus'' (stalked shaped cell) into ''Escherchia coli'' (rod shaped cell) to see if mreB will affect ''E. coli's'' cell shape in some way. We expect to see the ''E. coli'' cells lyse or change from their normal rod shape.  </div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td colspan="2" class="diff-lineno">Line 45:</td> <td colspan="2" class="diff-lineno">Line 47:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Verification testing by examining the physical cell shapes under a light microscope and sequence verification by sending a sample of cloned DNA to Iowa State.  </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Verification testing by examining the physical cell shapes under a light microscope and sequence verification by sending a sample of cloned DNA to Iowa State.  </div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">To see our full project description see initial project presentation: http://openwetware.org/wiki/Image:25_Recombinant_Presentation.pdf</del></div></td><td colspan="2"> </td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==References==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==References==</div></td></tr>  </table>

Bess E. Lippmann