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Team Tango Alpha
mreB Project Description
The goal of our project is to clone the gene, mreB, from Caulobacter crescentus (stalked shaped cell) into Escherchia coli (rod shaped cell) to see if mreB will affect E. coli's cell shape in some way. We expect to see the E. coli cells lyse or change from their normal rod shape.
MreB is a rod-shape determining gene that appears as bands or spirals encircling the cell. It is known to associate with mreC and penicillin-binding proteins to catalyze precursors for the peptidoglycan cell wall and correctly position polar bacterial proteins. It is also homologous of actin in eukaryotes.
Forward primer: the first 34 bases of our gene sequence. We also added in the Biobrick enzymes EcoR1 and Xba1 in front of the primer.
Reverse primer: the last 20 bases of our gene sequence. We also added in the Biobrick enzymes Pst1 and Spe1 in front of the primer.
Gitai, Z., & Yakhnina, A.A. (2012). The small protein mbiA interacts with mreB and modulates cell shape in caulobacter crescentus. Molecular Microbiology. doi: 10.1111/j.1365-2958.2012.08159.x
Jacobs-Wagner, C., et al. (2012). Osmolality dependent relocation of penicillin-binding protein PBP2 to the division site in caulobacter crescentus. Journal of Bacteriology. doi: 10.1128