840:153g:Projects/project25/2012/09/27

From OpenWetWare

< 840:153g:Projects | project25 | 2012 | 09(Difference between revisions)
Jump to: navigation, search
(Autocreate 2012/09/27 Entry for 840:153g:Projects/project25)
Current revision (15:55, 2 October 2012) (view source)
 
(5 intermediate revisions not shown.)
Line 1: Line 1:
-
Team Members
+
{|{{table}} width="800"
 +
|-
 +
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Cloning of mreB gene from ''Caulobacter crescentus''</span>
 +
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
 +
|-
 +
| colspan="2"|
 +
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
 +
==DNA Extraction/PCR==
-
    Name of student
+
On Tuesday, 9/25, we finished our DNA Extraction protocol and ran out the 'DNA' with gel Electrophoresis to see if we actually had any.  We ran a 100 bp plus Marker, our genome DNA, and our DNA digested with ECOR1.  This is a picture of the gel: [[http://openwetware.org/wiki/Image:2012-09-27_16-23-27_929.jpg]].  The first well is the marker, well 2 is whole DNA, and well 3 is digested DNA.  This shows us that we have about the appropriate size of DNA  and it is good to work with.  We also made our stock solutions of our extended primers.
-
    Name of student
+
-
Project Name and Description
+
[[Image:2012-09-27_16-23-27_929.jpg|400px]]
-
    Explain your experimental design here
+
On Thursday, 9/27, we planned out and executed our PCR reaction set. We ran out a negative control, primers without DNA template, and a positive control, plasmid 1A7 with VF2/VR primers which were provided by our instructor Dr. Axel.  We ran 3 sets of our DNA samples with different annealing temperatures.  1 set with 65°C, 1 with 60°C, and 1 with 55°C.  The reactions were to run overnight and stored at 4°C (in freezer) for the weekend.  We will run these out on a gel Tuesday, 10/2, to look at our results.
-
    List all the steps that are needed to complete your project
+
-
    Do not go into experimental details and don't list procedures. Just list the major steps necessary to complete your project
+
-
    Please also make yourself familiar with uploading pictures and *.ppt files
+
-
Important Results and Milestones
 
-
    keep track of your most important results and refer to the corresponding page in your notebook
+
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
-
    upload important pictures (don't forget to label them! Powerpoint is very convenient). Remember: these will become quite handy later in your summary report or final presentation. If you do label and upload the pictures as soon as you got them, your summary report can be written much more effortlessly (do you usually procrastinate? This is chance to do some work before hand that frees you up for finals week).  
+
|}
-
Recent changes
+
__NOTOC__
 +
[[category:OWWLabNotebookV1]]

Current revision

Cloning of mreB gene from Caulobacter crescentus Main project page
Previous entry      Next entry

DNA Extraction/PCR

On Tuesday, 9/25, we finished our DNA Extraction protocol and ran out the 'DNA' with gel Electrophoresis to see if we actually had any. We ran a 100 bp plus Marker, our genome DNA, and our DNA digested with ECOR1. This is a picture of the gel: [[1]]. The first well is the marker, well 2 is whole DNA, and well 3 is digested DNA. This shows us that we have about the appropriate size of DNA and it is good to work with. We also made our stock solutions of our extended primers.

On Thursday, 9/27, we planned out and executed our PCR reaction set. We ran out a negative control, primers without DNA template, and a positive control, plasmid 1A7 with VF2/VR primers which were provided by our instructor Dr. Axel. We ran 3 sets of our DNA samples with different annealing temperatures. 1 set with 65°C, 1 with 60°C, and 1 with 55°C. The reactions were to run overnight and stored at 4°C (in freezer) for the weekend. We will run these out on a gel Tuesday, 10/2, to look at our results.


Personal tools