Bess E. Lippmann at 21:43, 18 October 2012

Bess E. Lippmann — Thu, 18 Oct 2012 21:43:25 GMT

←Older revision		Revision as of 21:43, 18 October 2012
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-	\|style="background-color: #EEE"\|[[Image:owwnotebook_icon.png\|128px]]<span style="font-size:22px;"> ~~Project Name~~</span>	+	\|style="background-color: #EEE"\|[[Image:owwnotebook_icon.png\|128px]]<span style="font-size:22px;"> Cloning of mreB from ''Caulobacter crescentus'' into ''E. coli'' </span>
	\|style="background-color: #F2F2F2" align="center"\|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}\|0\|-11}}\|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}\|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}\|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}		\|style="background-color: #F2F2F2" align="center"\|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}\|0\|-11}}\|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}\|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}\|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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-	==~~Title here~~==	+	==Experiment 5 Preparation==

		+	This week we worked on preparing for plasmid transformation using our primary plasmid (PSB1A3) and our alternative plasmid (PSB2K3). We made LBA and LBA with ampicillin plates for our PSB1A3 plasmid. The PSB2K3 plates were previously made (with kanamycin) and supplied by our professor. The PSB2K3 plasmid has already been transformed; we will only need to streak it onto the plates when we come to that point with our PSB1A3 plasmid. Ampicillin selects for PSB1A3 and kanamycin selects for PSB2K3.

		+	We also performed extraction of PSB1A3 from Parts Registry 2011 Distribution Kit. This will allow us to start transformation next week.

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Bess E. Lippmann: Autocreate 2012/10/18 Entry for 840:153g:Projects/project25

Bess E. Lippmann — Thu, 18 Oct 2012 21:26:42 GMT

Autocreate 2012/10/18 Entry for 840:153g:Projects/project25

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project Name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Title here==


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[[category:OWWLabNotebookV1]]

840:153g:Projects/project25/2012/10/18 - Revision history

Bess E. Lippmann at 21:43, 18 October 2012

Bess E. Lippmann: Autocreate 2012/10/18 Entry for 840:153g:Projects/project25