840:153g:Projects/project25/2012/11/08: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Cloning of mreB from ''Caulobacter crescentus'' into ''E. coli''</span> | ||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
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== | ==Exp 6: Prepatory Digest, Purification, Electrophoresis. & Exp 7:Ligation of Insert and Vector== | ||
Tuesday: | |||
We ran a gel of our digested vector (part 1 and 2) and digested gene segment with a 100bp plus DNA ladder and FastRuler Low Range DNA Ladder. We expected to see our Gene segment and vector samples at the correct length and the wells be very clean. The intensity of the bands, in comparison with the FastRuler Low Range DNA Ladder, will give us an estimated amount of DNA per microliter. This will let us figure out our insert:vector ratios for ligation, which will ideally be 2:1. | |||
[[Image:2012-11-08 15.22.27.jpg|400px]] | |||
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Revision as of 14:31, 8 November 2012
 Cloning of mreB from Caulobacter crescentus into E. coli
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<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Exp 6: Prepatory Digest, Purification, Electrophoresis. & Exp 7:Ligation of Insert and VectorTuesday: We ran a gel of our digested vector (part 1 and 2) and digested gene segment with a 100bp plus DNA ladder and FastRuler Low Range DNA Ladder. We expected to see our Gene segment and vector samples at the correct length and the wells be very clean. The intensity of the bands, in comparison with the FastRuler Low Range DNA Ladder, will give us an estimated amount of DNA per microliter. This will let us figure out our insert:vector ratios for ligation, which will ideally be 2:1.
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