840:153g:Projects/project26/2012/10/18

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(DNA Extraction)
(DNA Extraction)
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==DNA Extraction==
==DNA Extraction==
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* The Gel on Tuesday didn't show any DNA from the PCR run last Thursday. Today and new DNA Extraction protocol was found. It was started with preparing buffers that were needed. Then the procedure was started with preparing a sample of skin with lysis buffer and proteinase K. The sample is to be on the heating block overnight and will be taken out friday at noon, and placed in the fridge for storage. The process will then be picked up on Tuesday.
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* The Gel on Tuesday didn't show any DNA from the PCR run last Thursday. Today and new DNA Extraction protocol was found. It was started with preparing buffers that were needed. Then the procedure was started with preparing a sample of skin with lysis buffer and proteinase K. The sample is to be on the heating block overnight and will be taken out friday at noon, and placed in the fridge for storage. The process will then be picked up on Tuesday.The far left lane is a 500 BP ladder with the row to the right of that being a positive conrtol. To the right of that is a negative control for Primer set 2. To the right of that DNA sample 2 with Primer sequence 2. To the right of that DNA sample 1 with primer sample 2. To the right of that we did another 500 BP ladder. To the right of that we did primer set 1 negative control. The next lane to the right is DNA sample 2 primer set 1. To the right of that is DNA sample 1 Primer set 1.
[[Image:!OCT15-PCR.tif]]
[[Image:!OCT15-PCR.tif]]

Revision as of 16:02, 23 October 2012

Cloning of Atrolysin A Main project page
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DNA Extraction

  • The Gel on Tuesday didn't show any DNA from the PCR run last Thursday. Today and new DNA Extraction protocol was found. It was started with preparing buffers that were needed. Then the procedure was started with preparing a sample of skin with lysis buffer and proteinase K. The sample is to be on the heating block overnight and will be taken out friday at noon, and placed in the fridge for storage. The process will then be picked up on Tuesday.The far left lane is a 500 BP ladder with the row to the right of that being a positive conrtol. To the right of that is a negative control for Primer set 2. To the right of that DNA sample 2 with Primer sequence 2. To the right of that DNA sample 1 with primer sample 2. To the right of that we did another 500 BP ladder. To the right of that we did primer set 1 negative control. The next lane to the right is DNA sample 2 primer set 1. To the right of that is DNA sample 1 Primer set 1.

Image:!OCT15-PCR.tif

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