840:153g:Projects/project26/2012/10/25

From OpenWetWare

< 840:153g:Projects | project26 | 2012 | 10(Difference between revisions)
Jump to: navigation, search
(Autocreate 2012/10/25 Entry for 840:153g:Projects/project26)
Current revision (11:53, 4 December 2012) (view source)
 
(5 intermediate revisions not shown.)
Line 1: Line 1:
{|{{table}} width="800"
{|{{table}} width="800"
|-
|-
-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
+
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Cloning of Atrolysin A from ''Crotalus atrox''</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|-
|-
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-
==Entry title==
+
==DNA Extraction==
-
* Insert content here...
+
This week we continued our new (second) DNA extraction procedure. Tuesday we were able to see DNA with the naked eye in the solution we had made. Thursday we then ran a gel on that sample in the hopes that DNA would be present. The gel on Thursday revealed:
 +
[[Image:10-25-12A.tif]]
 +
Of the three lanes, the lane on the left is sample 3 undigested, the middle lane is sample 1 digested, and the right lane is sample 3 digested. There are bands present in all three lanes which means that DNA is present. Sample 1 is the most concentrated and sample 3 is the most dilute one we have. The result showed up best on sample 3 so that is the one we are going to use for amplifying and the rest of the DNA will be stored at -80 degrees. 
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
|}
|}

Current revision

Cloning of Atrolysin A from Crotalus atrox Main project page
Previous entry      Next entry

DNA Extraction

This week we continued our new (second) DNA extraction procedure. Tuesday we were able to see DNA with the naked eye in the solution we had made. Thursday we then ran a gel on that sample in the hopes that DNA would be present. The gel on Thursday revealed:

Image:10-25-12A.tif

Of the three lanes, the lane on the left is sample 3 undigested, the middle lane is sample 1 digested, and the right lane is sample 3 digested. There are bands present in all three lanes which means that DNA is present. Sample 1 is the most concentrated and sample 3 is the most dilute one we have. The result showed up best on sample 3 so that is the one we are going to use for amplifying and the rest of the DNA will be stored at -80 degrees.

Personal tools