840:153g:Projects/project26/2012/11/01: Difference between revisions

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==Entry title==
==DNA Extraction==
* On Tuesday of this week we ran a PCR of our DNA sample. Thursday we checked it on a gel and there was a high concentration down on the lanes. We then realized we used the Stock Solution of our primers instead of our Working Solution. We then set up another PCR reaction using the Working Solution of primers. Then next Tuesday we will check it on a gel. We have a couple of things to try. One is to put our gene into a T-vector and get sequenced. The other is if the PCR works then we will try a PCR with the extended primers and try and put our gene into the PSB vector. This all depends on what we find on Tuesday.
* On Tuesday of this week we ran a PCR of our DNA sample. Thursday we checked it on a gel and there was a high concentration down on the lanes. We then realized we used the Stock Solution of our primers instead of our Working Solution. We then set up another PCR reaction using the Working Solution of primers. Then next Tuesday we will check it on a gel. We have a couple of things to try. One is to put our gene into a T-vector and get sequenced. The other is if the PCR works then we will try a PCR with the extended primers and try and put our gene into the PSB vector. This all depends on what we find on Tuesday.


Revision as of 15:10, 1 November 2012

Cloning of Atrolysin A <html><img src="/images/9/94/Report.png" border="0" /></html> DNA Extraction
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DNA Extraction

  • On Tuesday of this week we ran a PCR of our DNA sample. Thursday we checked it on a gel and there was a high concentration down on the lanes. We then realized we used the Stock Solution of our primers instead of our Working Solution. We then set up another PCR reaction using the Working Solution of primers. Then next Tuesday we will check it on a gel. We have a couple of things to try. One is to put our gene into a T-vector and get sequenced. The other is if the PCR works then we will try a PCR with the extended primers and try and put our gene into the PSB vector. This all depends on what we find on Tuesday.
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