840:153g:Projects/project26/2012/11/08

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(Autocreate 2012/11/08 Entry for 840:153g:Projects/project26)
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Cloning of Atrolysin A</span>
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|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main Project Page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
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==PCR ==
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* Insert content here...
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This week We used our working primers to try and make a PCR product. Our first trial was a fail so we Then added a higher concentration of DNA and lowered our annealing temps these we are hoping allow for a positive DNA results. We also this week isolated our plasmid from our vector. We ran the DNA on a gel and got to see if the DNA was present or not. If present we will then be able to add our bio brick parts and incorporate our DNA in the plasmid.

Revision as of 18:03, 8 November 2012

Cloning of Atrolysin A Main Project Page
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PCR

This week We used our working primers to try and make a PCR product. Our first trial was a fail so we Then added a higher concentration of DNA and lowered our annealing temps these we are hoping allow for a positive DNA results. We also this week isolated our plasmid from our vector. We ran the DNA on a gel and got to see if the DNA was present or not. If present we will then be able to add our bio brick parts and incorporate our DNA in the plasmid.


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