840:153g:Projects/project26/2012/11/08 - Revision history

Kayla Ann Ohrt at 18:12, 4 December 2012

2012-12-04T18:12:38Z

←Older revision		Revision as of 18:12, 4 December 2012
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	{\|{{table}} width="800"		{\|{{table}} width="800"
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-	\|style="background-color: #EEE"\|[[Image:owwnotebook_icon.png\|128px]]<span style="font-size:22px;"> Cloning of Atrolysin A</span>	+	\|style="background-color: #EEE"\|[[Image:owwnotebook_icon.png\|128px]]<span style="font-size:22px;"> Cloning of Atrolysin A from ''Crotalus atrox''</span>
	\|style="background-color: #F2F2F2" align="center"\|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}\|0\|-11}}\|Main Project Page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}\|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}\|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}		\|style="background-color: #F2F2F2" align="center"\|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}\|0\|-11}}\|Main Project Page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}\|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}\|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
	==PCR ==		==PCR ==
-	This week We used our working primers to try and make a PCR product. Our first trial ~~was a fail~~ so we ~~Then~~ added a higher concentration of DNA and lowered our annealing temps ~~these we~~ are hoping allow for a positive DNA ~~results~~. We also ~~this week~~ isolated our plasmid BBa_I0500 which is 1210 Bp long~~, from our vector~~. We ran the ~~DNA~~ on a gel and got to see if the DNA was present or not. ~~And there~~ was faint banding around 1200 bp which is ~~a good sign~~. If present we will then be able to add our bio brick parts and incorporate our DNA in the plasmid.	+	This week we used our working primers to try and make a PCR product. Our first trial failed so we then added a higher concentration of DNA and lowered our annealing temps. We are hoping allow for a positive DNA result. We also isolated our plasmid BBa_I0500 which is 1210 Bp long. We ran the sample on a gel and got to see if the DNA was present or not. There was faint banding around 1200 bp which is the size of the insert and banding at 4425 bp which is the size of the backbone. If present we will then be able to add our bio brick parts and incorporate our DNA in the plasmid.

	[[Image:11-6-12A.tif]]		[[Image:11-6-12A.tif]]

Kayla Ann Ohrt: /* PCR */

2012-11-13T21:08:45Z

PCR

←Older revision		Revision as of 21:08, 13 November 2012
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	Starting from the left: there is the positive control, negative control, E3, A3, Ladder, E2, A2, E1, A1. No bands showed up on the gel meaning that there was no amplification.		Starting from the left: there is the positive control, negative control, E3, A3, Ladder, E2, A2, E1, A1. No bands showed up on the gel meaning that there was no amplification.
		+
		+	[[Image:11-8-12A.tif]]
		+
		+	This gel is of the plasmid DNA. Starting from the left is: K4, K3, Ladder, K2, K1. The bands that showed up were light, but present. They showed up around the 1200 bp mark.

	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Kayla Ann Ohrt: /* PCR */

2012-11-13T21:05:12Z

PCR

←Older revision		Revision as of 21:05, 13 November 2012
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	This week We used our working primers to try and make a PCR product. Our first trial was a fail so we Then added a higher concentration of DNA and lowered our annealing temps these we are hoping allow for a positive DNA results. We also this week isolated our plasmid BBa_I0500 which is 1210 Bp long, from our vector. We ran the DNA on a gel and got to see if the DNA was present or not. And there was faint banding around 1200 bp which is a good sign. If present we will then be able to add our bio brick parts and incorporate our DNA in the plasmid.		This week We used our working primers to try and make a PCR product. Our first trial was a fail so we Then added a higher concentration of DNA and lowered our annealing temps these we are hoping allow for a positive DNA results. We also this week isolated our plasmid BBa_I0500 which is 1210 Bp long, from our vector. We ran the DNA on a gel and got to see if the DNA was present or not. And there was faint banding around 1200 bp which is a good sign. If present we will then be able to add our bio brick parts and incorporate our DNA in the plasmid.

		+	[[Image:11-6-12A.tif]]
		+
		+	Starting from the left: there is the positive control, negative control, E3, A3, Ladder, E2, A2, E1, A1. No bands showed up on the gel meaning that there was no amplification.

	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Kayla Ann Ohrt: /* PCR */

2012-11-08T23:12:15Z

PCR

←Older revision		Revision as of 23:12, 8 November 2012
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	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
	==PCR ==		==PCR ==
-	This week We used our working primers to try and make a PCR product. Our first trial was a fail so we Then added a higher concentration of DNA and lowered our annealing temps these we are hoping allow for a positive DNA results. We also this week isolated our plasmid BBa_I0500 from our vector. We ran the DNA on a gel and got to see if the DNA was present or not. If present we will then be able to add our bio brick parts and incorporate our DNA in the plasmid.	+	This week We used our working primers to try and make a PCR product. Our first trial was a fail so we Then added a higher concentration of DNA and lowered our annealing temps these we are hoping allow for a positive DNA results. We also this week isolated our plasmid BBa_I0500 which is 1210 Bp long, from our vector. We ran the DNA on a gel and got to see if the DNA was present or not. And there was faint banding around 1200 bp which is a good sign. If present we will then be able to add our bio brick parts and incorporate our DNA in the plasmid.

Kayla Ann Ohrt: /* PCR */

2012-11-08T22:30:09Z

PCR

←Older revision		Revision as of 22:30, 8 November 2012
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	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
	==PCR ==		==PCR ==
-	This week We used our working primers to try and make a PCR product. Our first trial was a fail so we Then added a higher concentration of DNA and lowered our annealing temps these we are hoping allow for a positive DNA results. We also this week isolated our plasmid from our vector. We ran the DNA on a gel and got to see if the DNA was present or not. If present we will then be able to add our bio brick parts and incorporate our DNA in the plasmid.	+	This week We used our working primers to try and make a PCR product. Our first trial was a fail so we Then added a higher concentration of DNA and lowered our annealing temps these we are hoping allow for a positive DNA results. We also this week isolated our plasmid BBa_I0500 from our vector. We ran the DNA on a gel and got to see if the DNA was present or not. If present we will then be able to add our bio brick parts and incorporate our DNA in the plasmid.

Kayla Ann Ohrt at 22:03, 8 November 2012

2012-11-08T22:03:25Z

←Older revision		Revision as of 22:03, 8 November 2012
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	{\|{{table}} width="800"		{\|{{table}} width="800"
	\|-		\|-
-	\|style="background-color: #EEE"\|[[Image:owwnotebook_icon.png\|128px]]<span style="font-size:22px;"> ~~Project name~~</span>	+	\|style="background-color: #EEE"\|[[Image:owwnotebook_icon.png\|128px]]<span style="font-size:22px;"> Cloning of Atrolysin A</span>
-	\|style="background-color: #F2F2F2" align="center"\|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}\|0\|-11}}\|Main ~~project page~~]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}\|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}\|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}	+	\|style="background-color: #F2F2F2" align="center"\|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}\|0\|-11}}\|Main Project Page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}\|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}\|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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	\| colspan="2"\|		\| colspan="2"\|
	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-	==~~Entry title~~==	+	==PCR ==
-	* Insert content here...	+	This week We used our working primers to try and make a PCR product. Our first trial was a fail so we Then added a higher concentration of DNA and lowered our annealing temps these we are hoping allow for a positive DNA results. We also this week isolated our plasmid from our vector. We ran the DNA on a gel and got to see if the DNA was present or not. If present we will then be able to add our bio brick parts and incorporate our DNA in the plasmid.

Kayla Ann Ohrt: Autocreate 2012/11/08 Entry for 840:153g:Projects/project26

2012-11-08T21:57:45Z

Autocreate 2012/11/08 Entry for 840:153g:Projects/project26

New page

{|{{table}} width="800"
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|-
| colspan="2"|

==Entry title==
* Insert content here...


|}

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[[category:OWWLabNotebookV1]]