840:153g:Projects/project26/2012/11/15: Difference between revisions
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Starting from the left to right: the first lane is the positive control, negative control, A5, A4, 1000 bp ladder, A3, A2, A1. The A1 sample was in the PCR at 65 degrees C, A2 at 61 degrees, A3 at 57 degrees, A4 at 48.8 degrees, and A5 and the controls were at 45 degrees | This is the gel of the PCR we did on our DNA to amplify our gene again. There were no bands appearing except for the positive control. Starting from the left to right: the first lane is the positive control, negative control, A5, A4, 1000 bp ladder, A3, A2, A1. The A1 sample was in the PCR at 65 degrees C, A2 at 61 degrees, A3 at 57 degrees, A4 at 48.8 degrees, and A5 and the controls were at 45 degrees. | ||
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Revision as of 14:13, 27 November 2012
 Cloning of Atrolysin A
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<html><img src="/images/9/94/Report.png" border="0" /></html> Main Project Page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
PCR with modificationsThis week we ran multiple gels to see if we could get banding of our DNA. In both gels we ran no banding was found. We ran with higher concentratiions of DNA. We went from 5ul to 8ul. We also had to change DNA samples which might have effected our results. We were running with DNA sample 3 which had the best banding, but had to switch to DNA samples 1 and 2. We also attempted to make a digestion of our plasmid DNA for future classes to use.
This is the gel of the PCR we did on our DNA to amplify our gene again. There were no bands appearing except for the positive control. Starting from the left to right: the first lane is the positive control, negative control, A5, A4, 1000 bp ladder, A3, A2, A1. The A1 sample was in the PCR at 65 degrees C, A2 at 61 degrees, A3 at 57 degrees, A4 at 48.8 degrees, and A5 and the controls were at 45 degrees. | |
