840:153g:Projects/project26/2012/11/15 - Revision history

Kayla Ann Ohrt at 17:20, 4 December 2012

Kayla Ann Ohrt — Tue, 04 Dec 2012 17:20:21 GMT

←Older revision		Revision as of 17:20, 4 December 2012
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	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
	==PCR with modifications==		==PCR with modifications==
-	This week we ran multiple gels to see if we could get banding of our DNA. In both gels we ran no banding was found. We ran with higher concentrations of DNA~~. We went from 5ul to 8ul. We also had to change DNA samples which might have effected our results. We were running with DNA sample 3 which had the best banding~~, ~~but had to switch to DNA samples 1~~ and 2. We also attempted to make a digestion of our plasmid DNA for future classes to use.	+	This week we ran multiple gels to see if we could get banding of our DNA. In both gels we ran no banding was found. We ran with higher concentrations of DNA, longer extension times, and lower PCR temperatures. We also attempted to make a digestion of our plasmid DNA for future classes to use.

	[[Image:11-13-12.tif]]		[[Image:11-13-12.tif]]

-	This is the gel of the PCR that we had ran 5 microliters of DNA and lowered our PCR temperatures 5 degrees overall. No bands were seen in the in the sample lanes except for A3. Starting from the left lane: Positive control, Negative control, A3 sample, 1000 Bp ladder, A2 sample, and A1 sample. A1 was at 60 degrees, A2 was at 50 degrees, A3 along with the Neg. and Pos. controls were at 45 degrees. The Positive control was seen at the 300 Bp mark.	+	This is the gel of the PCR that we had ran 5 microliters of DNA sample #3, and lowered our PCR temperatures 5 degrees overall. No bands were seen in the in the sample lanes except for A3. Starting from the left lane: Positive control, Negative control, A3 sample, 1000 Bp ladder, A2 sample, and A1 sample. A1 was at 60 degrees, A2 was at 50 degrees, A3 along with the Neg. and Pos. controls were at 45 degrees. The Positive control was seen at the 300 Bp mark.


	[[Image:nov 15A.tif]]		[[Image:nov 15A.tif]]

-	This is the gel of the PCR where we used 8 microliters of DNA instead of 5 microliters. We also made the extension time in the PCR for 5 minutes. to amplify our gene again. There were no bands appearing except for the positive control. Starting from the left to right: the first lane is the positive control (band seen at 300 Bp mark), negative control, A5, A4, 1000 Bp ladder, A3, A2, A1. The A1 sample was in the PCR at 65 degrees C, A2 at 61 degrees, A3 at 57 degrees, A4 at 48.8 degrees, and A5 and the controls were at 45 degrees.	+	This is the gel of the PCR where we used 8 microliters of DNA instead of 5 microliters. We had to use our samples #1 and #2 combined because we ran out of sample #3. We also made the extension time in the PCR for 5 minutes. to amplify our gene again. There were no bands appearing except for the positive control. Starting from the left to right: the first lane is the positive control (band seen at 300 Bp mark), negative control, A5, A4, 1000 Bp ladder, A3, A2, A1. The A1 sample was in the PCR at 65 degrees C, A2 at 61 degrees, A3 at 57 degrees, A4 at 48.8 degrees, and A5 and the controls were at 45 degrees.

	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Kayla Ann Ohrt at 17:16, 4 December 2012

Kayla Ann Ohrt — Tue, 04 Dec 2012 17:16:53 GMT

←Older revision		Revision as of 17:16, 4 December 2012
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	[[Image:11-13-12.tif]]		[[Image:11-13-12.tif]]

-	This is the gel of the PCR that we had ran 5 microliters of DNA and lowered our PCR temperatures 5 degrees overall. No bands were seen in the in the sample lanes except for A3.	+	This is the gel of the PCR that we had ran 5 microliters of DNA and lowered our PCR temperatures 5 degrees overall. No bands were seen in the in the sample lanes except for A3. Starting from the left lane: Positive control, Negative control, A3 sample, 1000 Bp ladder, A2 sample, and A1 sample. A1 was at 60 degrees, A2 was at 50 degrees, A3 along with the Neg. and Pos. controls were at 45 degrees. The Positive control was seen at the 300 Bp mark.


	[[Image:nov 15A.tif]]		[[Image:nov 15A.tif]]

-	This is the gel of the PCR where we used 8 microliters of DNA instead of 5 microliters. We also made the extension time in the PCR for 5 minutes. to amplify our gene again. There were no bands appearing except for the positive control. Starting from the left to right: the first lane is the positive control, negative control, A5, A4, 1000 Bp ladder, A3, A2, A1. The A1 sample was in the PCR at 65 degrees C, A2 at 61 degrees, A3 at 57 degrees, A4 at 48.8 degrees, and A5 and the controls were at 45 degrees.	+	This is the gel of the PCR where we used 8 microliters of DNA instead of 5 microliters. We also made the extension time in the PCR for 5 minutes. to amplify our gene again. There were no bands appearing except for the positive control. Starting from the left to right: the first lane is the positive control (band seen at 300 Bp mark), negative control, A5, A4, 1000 Bp ladder, A3, A2, A1. The A1 sample was in the PCR at 65 degrees C, A2 at 61 degrees, A3 at 57 degrees, A4 at 48.8 degrees, and A5 and the controls were at 45 degrees.

	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Kayla Ann Ohrt at 17:14, 4 December 2012

Kayla Ann Ohrt — Tue, 04 Dec 2012 17:14:15 GMT

←Older revision		Revision as of 17:14, 4 December 2012
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	{\|{{table}} width="800"		{\|{{table}} width="800"
	\|-		\|-
-	\|style="background-color: #EEE"\|[[Image:owwnotebook_icon.png\|128px]]<span style="font-size:22px;"> Cloning of Atrolysin A</span>	+	\|style="background-color: #EEE"\|[[Image:owwnotebook_icon.png\|128px]]<span style="font-size:22px;"> Cloning of Atrolysin A from ''Crotalus atrox''</span>
	\|style="background-color: #F2F2F2" align="center"\|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}\|0\|-11}}\|Main Project Page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}\|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}\|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}		\|style="background-color: #F2F2F2" align="center"\|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}\|0\|-11}}\|Main Project Page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}\|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}\|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
	\|-		\|-
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	This is the gel of the PCR that we had ran 5 microliters of DNA and lowered our PCR temperatures 5 degrees overall. No bands were seen in the in the sample lanes except for A3.		This is the gel of the PCR that we had ran 5 microliters of DNA and lowered our PCR temperatures 5 degrees overall. No bands were seen in the in the sample lanes except for A3.
		+

	[[Image:nov 15A.tif]]		[[Image:nov 15A.tif]]

Kayla Ann Ohrt at 17:13, 4 December 2012

Kayla Ann Ohrt — Tue, 04 Dec 2012 17:13:35 GMT

←Older revision		Revision as of 17:13, 4 December 2012
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	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
	==PCR with modifications==		==PCR with modifications==
-	This week we ran multiple gels to see if we could get banding of our DNA. In both gels we ran no banding was found. We ran with higher ~~concentratiions~~ of DNA. We went from 5ul to 8ul. We also had to change DNA samples which might have effected our results. We were running with DNA sample 3 which had the best banding, but had to switch to DNA samples 1 and 2. We also attempted to make a digestion of our plasmid DNA for future classes to use.	+	This week we ran multiple gels to see if we could get banding of our DNA. In both gels we ran no banding was found. We ran with higher concentrations of DNA. We went from 5ul to 8ul. We also had to change DNA samples which might have effected our results. We were running with DNA sample 3 which had the best banding, but had to switch to DNA samples 1 and 2. We also attempted to make a digestion of our plasmid DNA for future classes to use.
		+
		+	[[Image:11-13-12.tif]]
		+
		+	This is the gel of the PCR that we had ran 5 microliters of DNA and lowered our PCR temperatures 5 degrees overall. No bands were seen in the in the sample lanes except for A3.

	[[Image:nov 15A.tif]]		[[Image:nov 15A.tif]]

-	This is the gel of the PCR we ~~did on our~~ DNA to amplify our gene again. There were no bands appearing except for the positive control. Starting from the left to right: the first lane is the positive control, negative control, A5, A4, 1000 bp ladder, A3, A2, A1. The A1 sample was in the PCR at 65 degrees C, A2 at 61 degrees, A3 at 57 degrees, A4 at 48.8 degrees, and A5 and the controls were at 45 degrees.	+	This is the gel of the PCR where we used 8 microliters of DNA instead of 5 microliters. We also made the extension time in the PCR for 5 minutes. to amplify our gene again. There were no bands appearing except for the positive control. Starting from the left to right: the first lane is the positive control, negative control, A5, A4, 1000 Bp ladder, A3, A2, A1. The A1 sample was in the PCR at 65 degrees C, A2 at 61 degrees, A3 at 57 degrees, A4 at 48.8 degrees, and A5 and the controls were at 45 degrees.

	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Kayla Ann Ohrt at 21:13, 27 November 2012

Kayla Ann Ohrt — Tue, 27 Nov 2012 21:13:25 GMT

←Older revision		Revision as of 21:13, 27 November 2012
Line 11:		Line 11:
	[[Image:nov 15A.tif]]		[[Image:nov 15A.tif]]

-	Starting from the left to right: the first lane is the positive control, negative control, A5, A4, 1000 bp ladder, A3, A2, A1. The A1 sample was in the PCR at 65 degrees C, A2 at 61 degrees, A3 at 57 degrees, A4 at 48.8 degrees, and A5 and the controls were at 45 degrees~~. There were no bands that appeared except for the positive control~~.	+	This is the gel of the PCR we did on our DNA to amplify our gene again. There were no bands appearing except for the positive control. Starting from the left to right: the first lane is the positive control, negative control, A5, A4, 1000 bp ladder, A3, A2, A1. The A1 sample was in the PCR at 65 degrees C, A2 at 61 degrees, A3 at 57 degrees, A4 at 48.8 degrees, and A5 and the controls were at 45 degrees.

	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Kayla Ann Ohrt: /* PCR with modifications */

Kayla Ann Ohrt — Tue, 27 Nov 2012 21:11:52 GMT

PCR with modifications

←Older revision		Revision as of 21:11, 27 November 2012
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	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
	==PCR with modifications==		==PCR with modifications==
-	This week we ran ~~multipule~~ gels to see if we could get banding of our DNA. In both gels we ran no banding was found. We ran with higher concentratiions of DNA. We went from 5ul to 8ul. We also had to change DNA samples which might have effected our results. We were running with DNA sample 3 which had the best banding, but had to switch to DNA samples 1 and 2. We also attempted to make a digestion of our plasmid DNA for future classes to use.	+	This week we ran multiple gels to see if we could get banding of our DNA. In both gels we ran no banding was found. We ran with higher concentratiions of DNA. We went from 5ul to 8ul. We also had to change DNA samples which might have effected our results. We were running with DNA sample 3 which had the best banding, but had to switch to DNA samples 1 and 2. We also attempted to make a digestion of our plasmid DNA for future classes to use.

	[[Image:nov 15A.tif]]		[[Image:nov 15A.tif]]

Kayla Ann Ohrt at 21:10, 27 November 2012

Kayla Ann Ohrt — Tue, 27 Nov 2012 21:10:36 GMT

←Older revision		Revision as of 21:10, 27 November 2012
Line 9:		Line 9:
	This week we ran multipule gels to see if we could get banding of our DNA. In both gels we ran no banding was found. We ran with higher concentratiions of DNA. We went from 5ul to 8ul. We also had to change DNA samples which might have effected our results. We were running with DNA sample 3 which had the best banding, but had to switch to DNA samples 1 and 2. We also attempted to make a digestion of our plasmid DNA for future classes to use.		This week we ran multipule gels to see if we could get banding of our DNA. In both gels we ran no banding was found. We ran with higher concentratiions of DNA. We went from 5ul to 8ul. We also had to change DNA samples which might have effected our results. We were running with DNA sample 3 which had the best banding, but had to switch to DNA samples 1 and 2. We also attempted to make a digestion of our plasmid DNA for future classes to use.

		+	[[Image:nov 15A.tif]]
		+
		+	Starting from the left to right: the first lane is the positive control, negative control, A5, A4, 1000 bp ladder, A3, A2, A1. The A1 sample was in the PCR at 65 degrees C, A2 at 61 degrees, A3 at 57 degrees, A4 at 48.8 degrees, and A5 and the controls were at 45 degrees. There were no bands that appeared except for the positive control.

	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Kayla Ann Ohrt: /* PCR */

Kayla Ann Ohrt — Thu, 15 Nov 2012 21:54:40 GMT

PCR

←Older revision		Revision as of 21:54, 15 November 2012
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	\| colspan="2"\|		\| colspan="2"\|
	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-	==PCR==	+	==PCR with modifications==
-	This week we ran multipule gels to see if we could get banding. In both gels we ran no banding was found. We ran with higher concentratiions of DNA. We also had to change DNA samples which might have effected our results. We also attempted to make a digestion of our plasmid DNA for future classes to use.	+	This week we ran multipule gels to see if we could get banding of our DNA. In both gels we ran no banding was found. We ran with higher concentratiions of DNA. We went from 5ul to 8ul. We also had to change DNA samples which might have effected our results. We were running with DNA sample 3 which had the best banding, but had to switch to DNA samples 1 and 2. We also attempted to make a digestion of our plasmid DNA for future classes to use.

Kayla Ann Ohrt at 21:52, 15 November 2012

Kayla Ann Ohrt — Thu, 15 Nov 2012 21:52:32 GMT

←Older revision		Revision as of 21:52, 15 November 2012
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	{\|{{table}} width="800"		{\|{{table}} width="800"
	\|-		\|-
-	\|style="background-color: #EEE"\|[[Image:owwnotebook_icon.png\|128px]]<span style="font-size:22px;"> ~~Project name~~</span>	+	\|style="background-color: #EEE"\|[[Image:owwnotebook_icon.png\|128px]]<span style="font-size:22px;"> Cloning of Atrolysin A</span>
-	\|style="background-color: #F2F2F2" align="center"\|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}\|0\|-11}}\|Main ~~project page~~]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}\|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}\|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}	+	\|style="background-color: #F2F2F2" align="center"\|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}\|0\|-11}}\|Main Project Page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}\|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}\|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
	\|-		\|-
	\| colspan="2"\|		\| colspan="2"\|
	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-	==~~Entry title~~==	+	==PCR==
-	* Insert content here...	+	This week we ran multipule gels to see if we could get banding. In both gels we ran no banding was found. We ran with higher concentratiions of DNA. We also had to change DNA samples which might have effected our results. We also attempted to make a digestion of our plasmid DNA for future classes to use.

Kayla Ann Ohrt: Autocreate 2012/11/15 Entry for 840:153g:Projects/project26

Kayla Ann Ohrt — Thu, 15 Nov 2012 21:48:04 GMT

Autocreate 2012/11/15 Entry for 840:153g:Projects/project26

New page

{|{{table}} width="800"
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|-
| colspan="2"|

==Entry title==
* Insert content here...


|}

__NOTOC__
[[category:OWWLabNotebookV1]]