840:153g:Projects/project27/2012/10/02: Difference between revisions
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==Entry title== | ==Entry title== | ||
* Digested plasmid DNA that was extracted 9/27. DNA from each elution was digested, making 12 total digestions, two for each colony grown in liquid LB on 9/27 | * Digested plasmid DNA that was extracted 9/27. DNA from each elution was digested, making 12 total digestions, two for each colony grown in liquid LB on 9/27 | ||
* | * J61002 plasmid carrying J23100 promoter was digested with EcoR1 and Pst1 in order to extract the promoter and linearize the plasmid backbone | ||
* pSB1K3 and pSB1A7(+ control) were digested with EcoR1 in order to linearize the plasmid | * pSB1K3 and pSB1A7(+ control) were digested with EcoR1 in order to linearize the plasmid | ||
* Digested DNA was run on a gel | * Digested DNA was run on a gel to visualize the digested parts | ||
[[Image:Digest3-1.jpg|400px]] | |||
Revision as of 20:14, 18 October 2012
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