840:153g:Projects/project27/2012/10/09: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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==Entry title==
==Digestion Enzyme Test==
* Tested the digestion of Xba1 and Spe1 enzymes by incubating pSB1A7 plasmid in each enzyme for 10, 20, and 30 min.  Results were checked on a gel, compared to undigested plasmid.
* Tested the digestion of Xba1 and Spe1 enzymes by incubating pSB1A7 plasmid in each enzyme for 10, 20, and 30 min.  Results were checked on a gel, compared to undigested plasmid.
[[Image:Digest3.jpg|400px]]
Samples in lanes 3, 4, and 5 were incubated with Xba1 for 10, 20, and 30min, respectively.  Lane 6 contained undigested plasmid.  Samples in lanes 7, 8, and 9 were incubated with Spe1 for 10, 20, and 30min, respectively.  The gel shows good digestion by both enzymes at all time increments, as compared to circular plasmid, which moved a significantly shorter distance (Lane 6).




Latest revision as of 22:07, 26 September 2017

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Digestion Enzyme Test

  • Tested the digestion of Xba1 and Spe1 enzymes by incubating pSB1A7 plasmid in each enzyme for 10, 20, and 30 min. Results were checked on a gel, compared to undigested plasmid.

Samples in lanes 3, 4, and 5 were incubated with Xba1 for 10, 20, and 30min, respectively. Lane 6 contained undigested plasmid. Samples in lanes 7, 8, and 9 were incubated with Spe1 for 10, 20, and 30min, respectively. The gel shows good digestion by both enzymes at all time increments, as compared to circular plasmid, which moved a significantly shorter distance (Lane 6).


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