840:153g:Projects/project27/2012/10/11

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(Autocreate 2012/10/11 Entry for 840:153g:Projects/project27)
Current revision (22:02, 18 October 2012) (view source)
(RFP)
 
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==Entry title==
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==RFP==
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* Discovered mRFP gene in plasmids. Cultures containing pSB1K3 and those containing J61002 were red in color. A 1022bp insert in pSB1K3 contains the parts R0010, B0034, E1010, and B0015. A 845bp insert in J61002 contains J23100, E1010, and B0015.  We designed oligo-nucleotides, 27F_J23100 and 27R_J23100, in order to synthesize J23100 promoter.  We will digest out the promoter-RFP complex from the plasmid backbones and insert our target promoter
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RFP

  • Discovered mRFP gene in plasmids. Cultures containing pSB1K3 and those containing J61002 were red in color. A 1022bp insert in pSB1K3 contains the parts R0010, B0034, E1010, and B0015. A 845bp insert in J61002 contains J23100, E1010, and B0015. We designed oligo-nucleotides, 27F_J23100 and 27R_J23100, in order to synthesize J23100 promoter. We will digest out the promoter-RFP complex from the plasmid backbones and insert our target promoter
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