840:153g:Projects/project27/2012/11/06: Difference between revisions
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* Attempted the transformation of competent ''E. coli'' cells with the ligation products from 10/30 (J61002 plasmid backbone with J23100 insert, and pSB1K3 plasmid backbone with J23100 insert). After transformation, cells were plated on SB media containing the appropriate antibiotic, along with positive control plated on ampicillin and negative control plated on ampicillin, kanamycin, and no antibiotic. Untransformed cells were also plated on ampicillin, kanamycin, and no antibiotic. | * Attempted the transformation of competent ''E. coli'' cells with the ligation products from 10/30 (J61002 plasmid backbone with J23100 insert, and pSB1K3 plasmid backbone with J23100 insert). After transformation, cells were plated on SB media containing the appropriate antibiotic, along with positive control plated on ampicillin and negative control plated on ampicillin, kanamycin, and no antibiotic. Untransformed cells were also plated on ampicillin, kanamycin, and no antibiotic. | ||
* PCR results from 11/1 were checked on a gel. Results were inconclusive, as | * PCR results from 11/1 were checked on a gel. Results were inconclusive, as lanes showed smearing with expected band sizes being faint or none. Negative controls showed no results and positive control showed a distinct band at ~500bp, indicating that there was no error in the PCR procedure. | ||
[[Image:!mmoPCR2.jpg]] | [[Image:!mmoPCR2.jpg]] | ||
Lanes | Lanes | ||
1-3: mmoBZDC primers (target 2378bp) at 55, 60, 65 degrees, respectively | 1-3: mmoBZDC primers (target 2378bp) at 55, 60, 65 degrees, respectively | ||
4: negative control (no primers used) | 4: negative control (no primers used) |
Revision as of 14:26, 27 November 2012
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Transformation and PCR
Lanes 1-3: mmoBZDC primers (target 2378bp) at 55, 60, 65 degrees, respectively 4: negative control (no primers used) 5-8: mmoXY primers (target 2893bp) at 55, 60, 65 degrees, respectively 9: negative control (no primers used) 10: positive control (known template and primer set)
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