840:153g:Projects/project27/2012/11/13: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(fix raw html notebook nav) |
|||
(8 intermediate revisions by one other user not shown) | |||
Line 2: | Line 2: | ||
|- | |- | ||
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
== | ==Failed PCR...Again== | ||
* [[Image:MmoPCR1.jpg | * PCR products from 11/8 were checked on gel electrophoresis. Again, no distinct bands were shown by the products amplified by either primer set. The negative control, in which water was used instead of primers, also showed no amplification. The positive control, in which a known template and primer set was used, showed a distinct band at ~500bp. The two controls indicate that the PCR procedure was adequate for amplification and that there was no significant contamination. Lanes that included a 1:10 dilution of template DNA showed no amplification. Lanes that included 5μL template DNA and lanes with products with annealing temperatures of 45°C, 47°C, and 50°C all showed smearing with no distinct bands visible. | ||
[[Image:MmoPCR1.jpg]] | |||
Lanes | |||
1,6,13: Marker | |||
2: 5μL template, mmoXY primers, 60.7°C anneal | |||
3: 5μL template, mmoBZDC primers, 62°C anneal | |||
4: 1:10 template dilution, mmoXY primers, 60.7°C anneal | |||
5: 1:10 template dilution, mmoBZDC primers, 62°C anneal | |||
7-9: mmoXY primers, anneal of 45°C, 47°C, and 50°C, respectively | |||
10-12: mmoBZDC primers anneal of 45°C, 47°C, and 50°C, respectively | |||
14: negative control (no primers used) | |||
15: positive control (known template and primers) | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> |
Latest revision as of 22:17, 26 September 2017
Project name | Main project page Previous entry Next entry |
Failed PCR...Again
Lanes 1,6,13: Marker 2: 5μL template, mmoXY primers, 60.7°C anneal 3: 5μL template, mmoBZDC primers, 62°C anneal 4: 1:10 template dilution, mmoXY primers, 60.7°C anneal 5: 1:10 template dilution, mmoBZDC primers, 62°C anneal 7-9: mmoXY primers, anneal of 45°C, 47°C, and 50°C, respectively 10-12: mmoBZDC primers anneal of 45°C, 47°C, and 50°C, respectively 14: negative control (no primers used) 15: positive control (known template and primers) |