840:153g:Projects/project4/2009/02/19: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 11: Line 11:
* Some samples may be used to attempt to increase the amount of digested plasmid by repeating the digestion and purification steps, or to isolate the digested plasmid by extracting it from an agarose gel.
* Some samples may be used to attempt to increase the amount of digested plasmid by repeating the digestion and purification steps, or to isolate the digested plasmid by extracting it from an agarose gel.
* The samples containing both digested and undigested plasmids can be used in the next step of the experiment.
* The samples containing both digested and undigested plasmids can be used in the next step of the experiment.
''Derek, Oggie, and Katy''
* Genomic DNA was extracted from both subspecies of B. subtilis following a new protocol.
* There was a problem with the gel, but it appears that DNA was obtained.
* This will serve as the template DNA for the PCR.




<!-- ## Do not edit below this line unless you know what you are doing. ## -->
<!-- ## Do not edit below this line unless you know what you are doing. ## -->
|}
|}

Revision as of 13:02, 20 February 2009

MoldBusters' Journal <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Thursday 2/19

Josh and Casy

  • The digested plasmid DNA samples (5μL of each) were run on an agarose gel for 30 minutes.
  • The gel indicated that not all of the plasmid was digested, as there were two bands for each sample. This is ready for the aprE ligation, with about 720 ng of digested plasmid available.
  • Some samples may be used to attempt to increase the amount of digested plasmid by repeating the digestion and purification steps, or to isolate the digested plasmid by extracting it from an agarose gel.
  • The samples containing both digested and undigested plasmids can be used in the next step of the experiment.

Derek, Oggie, and Katy

  • Genomic DNA was extracted from both subspecies of B. subtilis following a new protocol.
  • There was a problem with the gel, but it appears that DNA was obtained.
  • This will serve as the template DNA for the PCR.


Retrieved from "https://openwetware.org/mediawiki/index.php?title=840:153g:Projects/project4/2009/02/19&oldid=287428"