840:153g:Projects/project4/2009/02/24: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Moldbusters</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> MoldBusters' Journal</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Tuesday 2/24==
==Tuesday 2/24==
''Josh and Casy''
''Josh and Casy''
* Today we started the isolation of the digested plasmid from the undigested plasmid.   
* Started the isolation of the digested plasmid from the undigested plasmid.   
*We ran 3 of our 4 DNA samples on a gel, then excised our target band from the Agarose gel and placed them in tubes and stored at -20°C until Thursday.   
* Ran 3 of 4 DNA samples on a gel, then excised our target band from the Agarose gel, and placed them in tubes and stored at -20°C until Thursday.   
*Thursday we will be using the QIAquick Gel Extraction Kit to isolate our digested plasmid.
* Thursday we will be using the QIAquick Gel Extraction Kit to isolate our digested plasmid.


"Derek, Oggie, and Katy"
''Derek, Oggie, and Katy''
*We diluted our primers today since we accidentally left out our previous ones overnight.
* We diluted our primers today since we accidentally left out our previous ones overnight.
*We also tried 5 different temperatures to find the optimum annealing temperature for our DNA.   
* We also tried 5 different temperatures to find the optimum annealing temperature for our DNA.   
*On Thursday we will check the outcome using gel electrophoresis.   
* On Thursday we will check the outcome using gel electrophoresis.   


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Revision as of 16:48, 24 February 2009

MoldBusters' Journal <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Tuesday 2/24

Josh and Casy

  • Started the isolation of the digested plasmid from the undigested plasmid.
  • Ran 3 of 4 DNA samples on a gel, then excised our target band from the Agarose gel, and placed them in tubes and stored at -20°C until Thursday.
  • Thursday we will be using the QIAquick Gel Extraction Kit to isolate our digested plasmid.

Derek, Oggie, and Katy

  • We diluted our primers today since we accidentally left out our previous ones overnight.
  • We also tried 5 different temperatures to find the optimum annealing temperature for our DNA.
  • On Thursday we will check the outcome using gel electrophoresis.
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