840:153g:Projects/project4/2009/03/11: Difference between revisions
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* The primers that have been used were determined to form hairpins and dimers with highly negative ΔG values. This was found to be primarily due to the sequences of the prefix and suffix. However, the following primers are proposed, which eliminate hairpins at operating temperature, and reduces the number of dimers that can be formed. The mutations, shown in parantheses, will not interfere with the amino acid sequence. | * The primers that have been used were determined to form hairpins and dimers with highly negative ΔG values. This was found to be primarily due to the sequences of the prefix and suffix. However, the following primers are proposed, which eliminate hairpins at operating temperature, and reduces the number of dimers that can be formed. The mutations, shown in parantheses, will not interfere with the amino acid sequence. | ||
Forward Primer: | Forward Primer: | ||
5’ TAGAATTCGCGGCCGCTTCTAG | 5’ TAGAATTCGCGGCCGCTTCTAG ATGAG(G)AGCAAAAAATTGTGG | ||
Reverse Primer: | Reverse Primer: |
Revision as of 14:56, 12 March 2009
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Wednesday 3/11Josh, Katy, and Oggie
Forward Primer: 5’ TAGAATTCGCGGCCGCTTCTAG ATGAG(G)AGCAAAAAATTGTGG Reverse Primer: 5’ ATCTGCAGCGGCCGCTACTAGTA TTATTGTGCAGC(A)GCTTGTAC
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