840:153g:Projects/project4/2009/04/02: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> MoldBusters' Journal</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
==Thursday 4/2: ''The Day of aprE Gene Amplification''==
* Insert content here...
''Josh and Casy''
* The PCR products were ran on a gel for 30 minutes. The gel showed two bands of approximately 1000 base pairs, according to the ladder. This is the size of the aprE gene. However, the presence of the second band was not expected and the cause is unknown.
* The gel was ran for another 30 minutes in order to separate the bands further, but they became blurred after running over the wells. The gel was discarded.
* The concentration of the template DNA did not have a large impact on the results. The temperature, however, was best at 50°C.
* The PCR success may be attributed to one of two factors. This is the first PCR using DNA from ''B. subtilis 168''. Also, the primers used in this PCR were mutated to prevent hairpin and dimer formation.
 
* Another PCR was setup for tuesday. This was setup with 0.1μL of DNA and at 50°C.
* Tuesday, the PCR product will be digested, ran on a gel, and the fragment(s) will be isolated and extracted from the gel.




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Revision as of 16:14, 2 April 2009

MoldBusters' Journal <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Thursday 4/2: The Day of aprE Gene Amplification

Josh and Casy

  • The PCR products were ran on a gel for 30 minutes. The gel showed two bands of approximately 1000 base pairs, according to the ladder. This is the size of the aprE gene. However, the presence of the second band was not expected and the cause is unknown.
  • The gel was ran for another 30 minutes in order to separate the bands further, but they became blurred after running over the wells. The gel was discarded.
  • The concentration of the template DNA did not have a large impact on the results. The temperature, however, was best at 50°C.
  • The PCR success may be attributed to one of two factors. This is the first PCR using DNA from B. subtilis 168. Also, the primers used in this PCR were mutated to prevent hairpin and dimer formation.
  • Another PCR was setup for tuesday. This was setup with 0.1μL of DNA and at 50°C.
  • Tuesday, the PCR product will be digested, ran on a gel, and the fragment(s) will be isolated and extracted from the gel.


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