840:153g:Projects/project4/2009/04/02: Difference between revisions
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* Tuesday, the PCR product will be digested, ran on a gel, and the fragment(s) will be isolated and extracted from the gel. | * Tuesday, the PCR product will be digested, ran on a gel, and the fragment(s) will be isolated and extracted from the gel. | ||
Derek and Katy | ''Derek and Katy'' | ||
* We did a PCR amplification of wintergreen using three temperatures, two concentrations, and two primers so that we could find the best combination to enhance amplification. | * We did a PCR amplification of wintergreen using three temperatures, two concentrations, and two primers so that we could find the best combination to enhance amplification. | ||
* We used 48, 52, and 56 degrees for the temperatures. | * We used 48, 52, and 56 degrees for the temperatures. | ||
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* We used concentrations 1.0mL and .1mL to see if there was too much DNA in our previous PCR's. | * We used concentrations 1.0mL and .1mL to see if there was too much DNA in our previous PCR's. | ||
* By doing this we hope to find combinations that yield better results than others. | * By doing this we hope to find combinations that yield better results than others. | ||
''Oggie'' | |||
* Performed several tests using PCR in hopes of troubleshooting and determining the cause of the nprE PCR failures | |||
* All results looked the same regardless of the forward primer differences | |||
* Tests involved samples containing just the reverse primer, and no primer at all | |||
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Revision as of 09:54, 14 April 2009
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Thursday 4/2: The Day of aprE Gene AmplificationJosh and Casy
Derek and Katy
Oggie
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