AB medium

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#Amino acids (check AA requirement of your [[E. coli genotypes | strain]]):  
#Amino acids (check AA requirement of your [[E. coli genotypes | strain]]):  
## 0.5% [[casamino acids]] (50ml of 10% sterile stock)  
## 0.5% [[casamino acids]] (50ml of 10% sterile stock)  
-
# 10μg/ml thiamine (1 ml of sterile 1 mg/ml stock)
+
# 10μg/ml thiamine (1 ml of sterile 10 mg/ml stock)
-
 
+
# 25μg/ml uridine (0.25 ml of sterile 10 mg/ml stock)(uridine makes E. coli K12 wildtypes as MG1655 of W3110 grow better because they are starving from pyrimidine <cite>Jensen-JB-1993</cite>.)
==References==
==References==
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#Clark-JMB-1967 Clark, DJ and Maaløe, O '''DNA replication and the division cycle in Escherichia coli''' Journal of Molecular Biology
#Clark-JMB-1967 Clark, DJ and Maaløe, O '''DNA replication and the division cycle in Escherichia coli''' Journal of Molecular Biology
Volume 23, Issue 1, 14 January 1967, Pages 99-112
Volume 23, Issue 1, 14 January 1967, Pages 99-112
 +
#Jensen-JB-1993 pmid=8501045
</biblio>
</biblio>

Revision as of 07:32, 27 March 2008

Contents

Purpose

  • AB medium is a minimal growth medium used for bacterial cultures. It has some changes compared to M9 medium and is supposed to course less problems with precipitation [1].

Recipe

5xA
for 1 liter AB medium
2 g (NH4)2SO4
6 g Na2HPO4
3 g KH2PO4
3 g NaCl
200 ml H2O

Prepare and autoclave.


1xB
for 1 liter AB medium
1 ml 0.1 M CaCl2 (sterile)
1 ml 1.0 M MgCl2 (sterile)
1 ml 0.003 M FeCl3 (sterile)
797 ml H2O (sterile)

Mix A and B. This makes AB. Do never add B salts to 5xA!


Different things can or should be added:

  1. Carbon source (has to be added):
    1. 0.2% glucose (10ml of 20% or 4ml of 50% sterile stock)
    2. 0.4% acetate (20ml of 20% sterile sodium-acetate stock)
  2. Amino acids (check AA requirement of your strain):
    1. 0.5% casamino acids (50ml of 10% sterile stock)
  3. 10μg/ml thiamine (1 ml of sterile 10 mg/ml stock)
  4. 25μg/ml uridine (0.25 ml of sterile 10 mg/ml stock)(uridine makes E. coli K12 wildtypes as MG1655 of W3110 grow better because they are starving from pyrimidine [2].)

References

  1. Clark, DJ and Maaløe, O DNA replication and the division cycle in Escherichia coli Journal of Molecular Biology Volume 23, Issue 1, 14 January 1967, Pages 99-112 [Clark-JMB-1967]
  2. Jensen KF. . pmid:8501045. PubMed HubMed [Jensen-JB-1993]

Contact

  • Please feel free to edit this page, add comments on the talk page or contact the page beginner Torsten Waldminghaus 06:12, 27 March 2008 (CDT).
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