AB medium
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Current revision (09:44, 11 October 2012) (view source) (→Recipe) |
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==Purpose== | ==Purpose== | ||
| - | *AB medium is a minimal growth medium used for bacterial cultures. It has some changes compared to [[M9 medium]] and is supposed to | + | *AB medium is a minimal growth medium used for bacterial cultures. It has some changes compared to [[M9 medium]] and is supposed to cause less problems with precipitation <cite>Clark-JMB-1967</cite>. |
==Recipe== | ==Recipe== | ||
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#Carbon source (has to be added): | #Carbon source (has to be added): | ||
| - | ## 0.2% glucose (10ml of 20% or 4ml of 50% sterile stock) | + | ## 0.2% [[glucose]] (10ml of 20% or 4ml of 50% sterile stock) |
| - | ## 0.4% acetate (20ml of 20% sterile sodium-acetate stock) | + | ## 0.4% acetate (20ml of 20% sterile [[sodium-acetate]] stock) |
#Amino acids (check AA requirement of your [[E. coli genotypes | strain]]): | #Amino acids (check AA requirement of your [[E. coli genotypes | strain]]): | ||
## 0.5% [[casamino acids]] (50ml of 10% sterile stock) | ## 0.5% [[casamino acids]] (50ml of 10% sterile stock) | ||
## For CM735 add 20 μg/ml methionine (10 ml of 2mg/ml stock), 20 μg/ml tryptophane (5 ml of 4mg/ml stock) and 25 μg/ml histidine (1 ml of 25 mg/ml stock); | ## For CM735 add 20 μg/ml methionine (10 ml of 2mg/ml stock), 20 μg/ml tryptophane (5 ml of 4mg/ml stock) and 25 μg/ml histidine (1 ml of 25 mg/ml stock); | ||
# 10μg/ml thiamine (1 ml of sterile 10 mg/ml stock) | # 10μg/ml thiamine (1 ml of sterile 10 mg/ml stock) | ||
| - | # 25μg/ml uridine ( | + | # 25μg/ml uridine (2.5 ml of sterile 10 mg/ml stock)(uridine makes E. coli K12 wildtypes as MG1655 of W3110 grow better because they are starving from pyrimidine <cite>Jensen-JB-1993</cite>.) |
==References== | ==References== | ||
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*Please feel free to edit this page, add comments on the talk page or contact the page beginner [[User:Torsten Waldminghaus|Torsten Waldminghaus]]. | *Please feel free to edit this page, add comments on the talk page or contact the page beginner [[User:Torsten Waldminghaus|Torsten Waldminghaus]]. | ||
| - | [[Category:Material]][[Category:Defined | + | [[Category:Material]][[Category:Defined Media]] |
Current revision
Contents |
Purpose
- AB medium is a minimal growth medium used for bacterial cultures. It has some changes compared to M9 medium and is supposed to cause less problems with precipitation [1].
Recipe
| for 1 liter AB medium |
|---|
| 2 g (NH4)2SO4 |
| 6 g Na2HPO4 |
| 3 g KH2PO4 |
| 3 g NaCl |
| 200 ml H2O |
Prepare and autoclave.
| for 1 liter AB medium |
|---|
| 1 ml 0.1 M CaCl2 (sterile) |
| 1 ml 1.0 M MgCl2 (sterile) |
| 1 ml 0.003 M FeCl3 (sterile) |
| 797 ml H2O (sterile) |
Mix 200ml 5xA and 800ml B to get 1 liter AB. Do never add B salts to 5xA!
If you want agar-plates add 16g agar to 1xB, autoclave and than add 5xA.
Different things can or should be added:
- Carbon source (has to be added):
- 0.2% glucose (10ml of 20% or 4ml of 50% sterile stock)
- 0.4% acetate (20ml of 20% sterile sodium-acetate stock)
- Amino acids (check AA requirement of your strain):
- 0.5% casamino acids (50ml of 10% sterile stock)
- For CM735 add 20 μg/ml methionine (10 ml of 2mg/ml stock), 20 μg/ml tryptophane (5 ml of 4mg/ml stock) and 25 μg/ml histidine (1 ml of 25 mg/ml stock);
- 10μg/ml thiamine (1 ml of sterile 10 mg/ml stock)
- 25μg/ml uridine (2.5 ml of sterile 10 mg/ml stock)(uridine makes E. coli K12 wildtypes as MG1655 of W3110 grow better because they are starving from pyrimidine [2].)
References
- Clark, DJ and Maaløe, O DNA replication and the division cycle in Escherichia coli Journal of Molecular Biology Volume 23, Issue 1, 14 January 1967, Pages 99-112 abstract
- Jensen KF. . pmid:8501045.
Contact
- Please feel free to edit this page, add comments on the talk page or contact the page beginner Torsten Waldminghaus.
