ATc

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anhydrotetracycline (ATc) is a tetracycline analog, showing increased (30x) affinity for tet repressor. 100 ng/mL gives induction at half maximum.
anhydrotetracycline (ATc) is a tetracycline analog, showing increased (30x) affinity for tet repressor. 100 ng/mL gives induction at half maximum.
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==Preparing working concentration==
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According to Nallamsetty and Waugh (2007):
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<pre>
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Anhydrotetracycline Prepare a 1,000 times stock solution by dissolving in 50% ethanol at 100 ug/ml. Store in a foil-covered tube at -20 °C.
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</pre>
==References==
==References==
[http://www.ncbi.nlm.nih.gov/entrez/utils/lofref.fcgi?PrId=3001&uid=8652521&db=pubmed&url=http://dx.doi.org/10.1021/bi952683e Tetracycline analogs affecting binding to Tn10-Encoded Tet repressor trigger the same mechanism of induction.]<br>
[http://www.ncbi.nlm.nih.gov/entrez/utils/lofref.fcgi?PrId=3001&uid=8652521&db=pubmed&url=http://dx.doi.org/10.1021/bi952683e Tetracycline analogs affecting binding to Tn10-Encoded Tet repressor trigger the same mechanism of induction.]<br>
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[http://www.nature.com/nprot/journal/v2/n2/full/nprot.2007.50.html#a1] <br>
[[Category:Material]] [[Category:Chemical]]
[[Category:Material]] [[Category:Chemical]]

Revision as of 00:29, 24 February 2008

anhydrotetracycline (ATc) is a tetracycline analog, showing increased (30x) affinity for tet repressor. 100 ng/mL gives induction at half maximum.

Preparing working concentration

According to Nallamsetty and Waugh (2007):


Anhydrotetracycline Prepare a 1,000 times stock solution by dissolving in 50% ethanol at 100 ug/ml. Store in a foil-covered tube at -20 °C.

References

Tetracycline analogs affecting binding to Tn10-Encoded Tet repressor trigger the same mechanism of induction.

[1]

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