ATc: Difference between revisions
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Anhydrotetracycline Prepare a 1,000 times stock solution by dissolving in 50% ethanol at 100 ug/ml. Store in a foil-covered tube at -20 °C. | Anhydrotetracycline Prepare a 1,000 times stock solution by dissolving | ||
in 50% ethanol at 100 ug/ml. Store in a foil-covered tube at -20 °C. | |||
</pre> | </pre> | ||
==References== | ==References== | ||
Revision as of 22:30, 23 February 2008
anhydrotetracycline (ATc) is a tetracycline analog, showing increased (30x) affinity for tet repressor. 100 ng/mL gives induction at half maximum.
Preparing working concentration
According to Nallamsetty and Waugh (2007):
Anhydrotetracycline Prepare a 1,000 times stock solution by dissolving in 50% ethanol at 100 ug/ml. Store in a foil-covered tube at -20 °C.
