ATc - Revision history

Janet B. Matsen: /* Preparing working concentration */

2012-05-18T01:37:07Z

Preparing working concentration

←Older revision		Revision as of 01:37, 18 May 2012
Line 5:		Line 5:
	<pre>		<pre>

-	Anhydrotetracycline Prepare a 1,000 times stock solution:	+	Anhydrotetracycline:
-	~~prepare a~~ 100 ug/mL solution in 50% ethanol. Store in a foil-covered tube at -20 °C.	+	Prepare a 1,000 times stock solution:
		+	100 ug/mL solution in 50% ethanol. Store in a foil-covered tube at -20 °C.

	</pre>		</pre>

Janet B. Matsen: /* Preparing working concentration */

2012-05-18T01:36:47Z

Preparing working concentration

←Older revision		Revision as of 01:36, 18 May 2012
Line 5:		Line 5:
	<pre>		<pre>

-	Anhydrotetracycline Prepare a 1,000 times stock solution ~~by dissolving~~	+	Anhydrotetracycline Prepare a 1,000 times stock solution:
-	in 50% ethanol ~~at 100 ug/ml~~. Store in a foil-covered tube at -20 °C.	+	prepare a 100 ug/mL solution in 50% ethanol. Store in a foil-covered tube at -20 °C.

	</pre>		</pre>

-	MW = 468.22 g/mole. Thus 1X for the recipe above = 214 nM.	+	MW = 468.22 g/mole. Thus 1X for the recipe above = 214 nM.

	==References==		==References==

Janet B. Matsen at 22:06, 13 April 2012

2012-04-13T22:06:06Z

←Older revision		Revision as of 22:06, 13 April 2012
Line 9:		Line 9:

	</pre>		</pre>
		+
		+	MW = 468.22 g/mole. Thus 1X for the recipe above = 214 nM.

	==References==		==References==

Wmholtz at 20:27, 1 March 2009

2009-03-01T20:27:29Z

←Older revision		Revision as of 20:27, 1 March 2009
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-	anhydrotetracycline (ATc) is a tetracycline analog, showing increased (30x) affinity for tet repressor. ~~100~~ ng/mL gives induction at half maximum.	+	anhydrotetracycline (ATc) is a tetracycline analog, showing increased (30x) affinity for tet repressor. 42 ng/mL gives induction at half maximum according to an in vitro assay.

	==Preparing working concentration==		==Preparing working concentration==

Zsun: /* Preparing working concentration */

2008-02-24T05:30:08Z

Preparing working concentration

←Older revision		Revision as of 05:30, 24 February 2008
Line 5:		Line 5:
	<pre>		<pre>

-	Anhydrotetracycline Prepare a 1,000 times stock solution by dissolving in 50% ethanol at 100 ug/ml. Store in a foil-covered tube at -20 °C.	+	Anhydrotetracycline Prepare a 1,000 times stock solution by dissolving
		+	in 50% ethanol at 100 ug/ml. Store in a foil-covered tube at -20 °C.

	</pre>		</pre>
		+
	==References==		==References==

Zsun at 05:29, 24 February 2008

2008-02-24T05:29:59Z

←Older revision		Revision as of 05:29, 24 February 2008
Line 1:		Line 1:
	anhydrotetracycline (ATc) is a tetracycline analog, showing increased (30x) affinity for tet repressor. 100 ng/mL gives induction at half maximum.		anhydrotetracycline (ATc) is a tetracycline analog, showing increased (30x) affinity for tet repressor. 100 ng/mL gives induction at half maximum.

		+	==Preparing working concentration==
		+	According to Nallamsetty and Waugh (2007):
		+	<pre>
		+
		+	Anhydrotetracycline Prepare a 1,000 times stock solution by dissolving in 50% ethanol at 100 ug/ml. Store in a foil-covered tube at -20 °C.
		+
		+	</pre>
	==References==		==References==

	[http://www.ncbi.nlm.nih.gov/entrez/utils/lofref.fcgi?PrId=3001&uid=8652521&db=pubmed&url=http://dx.doi.org/10.1021/bi952683e Tetracycline analogs affecting binding to Tn10-Encoded Tet repressor trigger the same mechanism of induction.]<br>		[http://www.ncbi.nlm.nih.gov/entrez/utils/lofref.fcgi?PrId=3001&uid=8652521&db=pubmed&url=http://dx.doi.org/10.1021/bi952683e Tetracycline analogs affecting binding to Tn10-Encoded Tet repressor trigger the same mechanism of induction.]<br>
		+
		+	[http://www.nature.com/nprot/journal/v2/n2/full/nprot.2007.50.html#a1] <br>

	[[Category:Material]] [[Category:Chemical]]		[[Category:Material]] [[Category:Chemical]]

Reshma P. Shetty at 17:28, 19 October 2007

2007-10-19T17:28:27Z

←Older revision		Revision as of 17:28, 19 October 2007
Line 4:		Line 4:

	[http://www.ncbi.nlm.nih.gov/entrez/utils/lofref.fcgi?PrId=3001&uid=8652521&db=pubmed&url=http://dx.doi.org/10.1021/bi952683e Tetracycline analogs affecting binding to Tn10-Encoded Tet repressor trigger the same mechanism of induction.]<br>		[http://www.ncbi.nlm.nih.gov/entrez/utils/lofref.fcgi?PrId=3001&uid=8652521&db=pubmed&url=http://dx.doi.org/10.1021/bi952683e Tetracycline analogs affecting binding to Tn10-Encoded Tet repressor trigger the same mechanism of induction.]<br>
		+
		+	[[Category:Material]] [[Category:Chemical]]

Melissali at 18:20, 17 July 2006

2006-07-17T18:20:52Z

←Older revision		Revision as of 18:20, 17 July 2006
Line 1:		Line 1:
-	~~Tetracycline~~ analog, showing increased (30x) affinity for tet repressor. 100 ng/mL gives induction at half maximum.	+	anhydrotetracycline (ATc) is a tetracycline analog, showing increased (30x) affinity for tet repressor. 100 ng/mL gives induction at half maximum.

	==References==		==References==

	[http://www.ncbi.nlm.nih.gov/entrez/utils/lofref.fcgi?PrId=3001&uid=8652521&db=pubmed&url=http://dx.doi.org/10.1021/bi952683e Tetracycline analogs affecting binding to Tn10-Encoded Tet repressor trigger the same mechanism of induction.]<br>		[http://www.ncbi.nlm.nih.gov/entrez/utils/lofref.fcgi?PrId=3001&uid=8652521&db=pubmed&url=http://dx.doi.org/10.1021/bi952683e Tetracycline analogs affecting binding to Tn10-Encoded Tet repressor trigger the same mechanism of induction.]<br>

Jason R. Kelly at 23:04, 19 July 2005

2005-07-19T23:04:02Z

←Older revision		Revision as of 23:04, 19 July 2005
Line 1:		Line 1:
	Tetracycline analog, showing increased (30x) affinity for tet repressor. 100 ng/mL gives induction at half maximum.		Tetracycline analog, showing increased (30x) affinity for tet repressor. 100 ng/mL gives induction at half maximum.
-	~~<br>~~	+
		+	==References==
		+
	[http://www.ncbi.nlm.nih.gov/entrez/utils/lofref.fcgi?PrId=3001&uid=8652521&db=pubmed&url=http://dx.doi.org/10.1021/bi952683e Tetracycline analogs affecting binding to Tn10-Encoded Tet repressor trigger the same mechanism of induction.]<br>		[http://www.ncbi.nlm.nih.gov/entrez/utils/lofref.fcgi?PrId=3001&uid=8652521&db=pubmed&url=http://dx.doi.org/10.1021/bi952683e Tetracycline analogs affecting binding to Tn10-Encoded Tet repressor trigger the same mechanism of induction.]<br>

Josh K. Michener at 21:18, 19 July 2005

2005-07-19T21:18:18Z

New page

Tetracycline analog, showing increased (30x) affinity for tet repressor. 100 ng/mL gives induction at half maximum.
<br>
[http://www.ncbi.nlm.nih.gov/entrez/utils/lofref.fcgi?PrId=3001&uid=8652521&db=pubmed&url=http://dx.doi.org/10.1021/bi952683e Tetracycline analogs affecting binding to Tn10-Encoded Tet repressor trigger the same mechanism of induction.]<br>