AU Biomaterials Design Lab:Equipment/LS55 PerkinElmer - Revision history

Dhea Patel: /* Specifications */

Dhea Patel — Fri, 07 Dec 2012 19:00:11 GMT

Specifications

←Older revision		Revision as of 19:00, 7 December 2012
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	*A single position water thermostattable holder for 10mm cuvettes.		*A single position water thermostattable holder for 10mm cuvettes.
	'''Physical'''<br>		'''Physical'''<br>
-	*Dimensions (W×D×H): <br>	+	*Dimensions (W×D×H): approximately, 2.5' X 2' X 8"<br>

	<br>		<br>

Dhea Patel: /* Description */

Dhea Patel — Fri, 07 Dec 2012 18:59:23 GMT

Description

←Older revision		Revision as of 18:59, 7 December 2012
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	==Description==		==Description==
-	[[Image]]	+	[[Image:LS55_Perkin_Elmer_Fluorimeter.jpg\|400px]]
	*Perkin Elmer LS 55 Fluorescence Spectrometer		*Perkin Elmer LS 55 Fluorescence Spectrometer
	*This is a 0.1nm resolution, compact spectrophotometer that uses the windows-compatible FL WinLab™ software that can measure fluorescence, phosphorescence and bio- and chemi-luminescence using a pulsed Xenon lamp source.		*This is a 0.1nm resolution, compact spectrophotometer that uses the windows-compatible FL WinLab™ software that can measure fluorescence, phosphorescence and bio- and chemi-luminescence using a pulsed Xenon lamp source.

Dhea Patel: /* Use */

Dhea Patel — Thu, 06 Dec 2012 20:30:03 GMT

Use

←Older revision		Revision as of 20:30, 6 December 2012
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	*After the sensor is calibrated using the FL WinLab LS55 Status application, the application will automatically record the temperature at the start of data collection in the result dataset header.		*After the sensor is calibrated using the FL WinLab LS55 Status application, the application will automatically record the temperature at the start of data collection in the result dataset header.
	*Using the Read application, the intensity, polarization, or anisotropy data can be collected simultaneously with the temperature and saved as a spreadsheet.		*Using the Read application, the intensity, polarization, or anisotropy data can be collected simultaneously with the temperature and saved as a spreadsheet.
-	*The event marker button on the front plate accessory can be used for identification of times when reagents are added to the cuvette.	+	*The event marker button on the front plate accessory can be used for identification of times when reagents are added to the cuvette.
-		+
-		+
-		+
-	*''Include any relevant links to [[Protocols\|OWW protocols]]''	+
-	*''Include any relevant links to the manufacturer's protocols''	+
-	*Note to users: you may note any troubleshooting or log your use on the [[{{TALKPAGENAME}}\|talk page]].	+

	==Safety==		==Safety==

Dhea Patel: /* Use */

Dhea Patel — Thu, 06 Dec 2012 20:29:29 GMT

Use

←Older revision		Revision as of 20:29, 6 December 2012
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	==Use==		==Use==
-	*''''Turning on the Instrument'''	+
-	**There is a on/off switch located on the left-hand side of the instrument's panel.	+	*There is a on/off switch located on the left-hand side of the instrument's panel.
-	**When the switch is turned on, the neon lamp on the front lower left-hand side of the instrument is lit.	+	*When the switch is turned on, the neon lamp on the front lower left-hand side of the instrument is lit.
-	**It takes about 1 minutes to initialize.	+	*It takes about 1 minutes to initialize.
-	**The instrument should be completely switched on before switching on the computer.	+	*The instrument should be completely switched on before switching on the computer.
-	** Switch on the PC	+	* Switch on the PC
-	**When Windows has completely loaded, start the FL WinLab software.	+	*When Windows has completely loaded, start the FL WinLab software.
		+	*After instruments calibrated and self checked, proceed to toolbar to select methods.
		+	*Samples can be placed into the sample compartment.
		+	*The door to sample compartment should be closed before samples are run.
		+
		+	'''Single Position Cellholder (Standard)'''
		+	*This cell holder requires no alignment or set up.
		+	*It holds 12.5mm square (10 mm pathlength) cuvettes and can be thermostatted using circulated water.
		+
		+	'''Stirred Single Position Cellholder'''
		+	*This cell holder requires no alignment or set up.
		+	*It holds 12.5mm square (10 mm pathlength) cuvettes and can be thermostatted using circulated water.
		+	*The stirrer status can be set to low, high, or off by clicking the accessory icon.
		+
		+	'''Stirred Four-Position Chellchanger'''
		+	*This cell holder requires no alignment or set up.
		+	*It holds 12.5mm square (10 mm pathlength) cuvettes and can be thermostatted using circulated water.
		+
		+	'''Biokinetics Accessory'''
		+	*This cell holder requires no alignment or set up.
		+	*It holds 12.5mm square (10 mm pathlength) cuvettes and can be thermostatted using circulated water.
		+	*The temperature sensor located in the block of the cellholder will report the temperature of the sample.
		+	*After the sensor is calibrated using the FL WinLab LS55 Status application, the application will automatically record the temperature at the start of data collection in the result dataset header.
		+	*Using the Read application, the intensity, polarization, or anisotropy data can be collected simultaneously with the temperature and saved as a spreadsheet.
		+	*The event marker button on the front plate accessory can be used for identification of times when reagents are added to the cuvette.
		+
		+

	*''Include any relevant links to [[Protocols\|OWW protocols]]''		*''Include any relevant links to [[Protocols\|OWW protocols]]''

Dhea Patel: /* Use */

Dhea Patel — Thu, 06 Dec 2012 18:34:49 GMT

Use

←Older revision		Revision as of 18:34, 6 December 2012
Line 30:		Line 30:

	==Use==		==Use==
-	*''~~How does one use this instrument.~~''	+	*''''Turning on the Instrument'''
		+	**There is a on/off switch located on the left-hand side of the instrument's panel.
		+	**When the switch is turned on, the neon lamp on the front lower left-hand side of the instrument is lit.
		+	**It takes about 1 minutes to initialize.
		+	**The instrument should be completely switched on before switching on the computer.
		+	** Switch on the PC
		+	**When Windows has completely loaded, start the FL WinLab software.
		+
	*''Include any relevant links to [[Protocols\|OWW protocols]]''		*''Include any relevant links to [[Protocols\|OWW protocols]]''
	*''Include any relevant links to the manufacturer's protocols''		*''Include any relevant links to the manufacturer's protocols''

Dhea Patel: /* Safety */

Dhea Patel — Thu, 06 Dec 2012 18:24:48 GMT

Safety

←Older revision		Revision as of 18:24, 6 December 2012
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	==Safety==		==Safety==
-	*'~~'Are there any safety considerations~~ for ~~this~~ instrument~~?''~~	+	*The equipment must be grounded.
		+	*There are three Caution labels on the instrument, indicating that the user's manual needs to be consulted.
		+	**In the sample area, the caution label warns users of the potential for eye damage caused by staring into the beam in the sample compartment.
		+	**Near the mains inlet fuse, the caution label warns users that the operator replaceable fuse is a CSA approved fuse, not an IEC fuse.
		+	**At the front left of the instrument, by the hand screw that fixes the top cover, the caution label warns that removing the cover can expose hazardous voltages.
		+	***The caution label is coupled with a high voltage label.
		+	*The other High voltage label is located inside the instrument on top of the monochromator cover.

	==Specifications==		==Specifications==

Dhea Patel: /* References */

Dhea Patel — Thu, 06 Dec 2012 14:58:10 GMT

References

←Older revision		Revision as of 14:58, 6 December 2012
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	*[http://www.perkinelmer.com/Catalog/Product/ID/L2250107 LS 55 Fluorescence Spectrometer, 230V]		*[http://www.perkinelmer.com/Catalog/Product/ID/L2250107 LS 55 Fluorescence Spectrometer, 230V]
	*[http://www.perkinelmer.com/CMSResources/Images/44-74453BRO_LS-55andLS-45FluorescenceSpectrophotometer.pdf LS 55 Manual]		*[http://www.perkinelmer.com/CMSResources/Images/44-74453BRO_LS-55andLS-45FluorescenceSpectrophotometer.pdf LS 55 Manual]
		+	*[http://www.perkinelmer.co.kr/files/LS%2055%20Users%20Guide%20-%2009934436.pdf LS55 User Manual]

	==Useful links==		==Useful links==

Dhea Patel: /* Specifications */

Dhea Patel — Thu, 06 Dec 2012 14:56:42 GMT

Specifications

←Older revision		Revision as of 14:56, 6 December 2012
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	*A single position water thermostattable holder for 10mm cuvettes.		*A single position water thermostattable holder for 10mm cuvettes.
	'''Physical'''<br>		'''Physical'''<br>
-	*Dimensions (W×D×H): ~~450(W) x 490(D) x 270(H) and Weight is 15kg~~<br>	+	*Dimensions (W×D×H): <br>
-	*Memory: USB memory, Saved as text and UVPC file<br>	+
	<br>		<br>

Dhea Patel: /* Specifications */

Dhea Patel — Thu, 06 Dec 2012 14:54:48 GMT

Specifications

←Older revision		Revision as of 14:54, 6 December 2012
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	'''Scanning speed'''		'''Scanning speed'''
	*Increments: 1nm		*Increments: 1nm
-	*Rate: 10-1500 nm/min	+	*Rate: 10-1500 nm/min<br>
	'''Sensitivity'''		'''Sensitivity'''
-	*Signal to Noise: 500:1 rms, using the Raman band of water with excitation at 350 nm~~, exciat~~	+	*Signal to Noise: 500:1 rms
		+	**using the Raman band of water with excitation at 350 nm
		+	** excitation and emission bandpass: 10nm<br>
		+	'''Standard Cellholder'''
		+	*A single position water thermostattable holder for 10mm cuvettes.
	'''Physical'''<br>		'''Physical'''<br>
	*Dimensions (W×D×H): 450(W) x 490(D) x 270(H) and Weight is 15kg<br>		*Dimensions (W×D×H): 450(W) x 490(D) x 270(H) and Weight is 15kg<br>

Dhea Patel: /* Specifications */

Dhea Patel — Thu, 06 Dec 2012 14:52:43 GMT

Specifications

←Older revision		Revision as of 14:52, 6 December 2012
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	**Emission:		**Emission:
	***Range: 200nm-650nm with standard photomultiplier with zero order selectable; 200-900m with optional R928 photomultiplier		***Range: 200nm-650nm with standard photomultiplier with zero order selectable; 200-900m with optional R928 photomultiplier
-	***Spectral bandpass:2.5-20.0nm, selected in 0.1nm increments<br>	+	***Spectral bandpass:2.5-20.0nm, selected in 0.1nm increments
		+	***Filters:
		+	****Computer selectable cut-off (high pass) at 290, 350, 390, 430, and 515nm
		+	****A blank that acts as a shutter
		+	****A 1% T attenuator
		+	****Clear beam position<br>
	'''Phosphorescence Mode'''		'''Phosphorescence Mode'''
	*Variable delay and gate times		*Variable delay and gate times
	*Minimum total period: 12.0ms (50Hz) <br>		*Minimum total period: 12.0ms (50Hz) <br>
-		+	'''Scanning speed'''
		+	*Increments: 1nm
		+	*Rate: 10-1500 nm/min
		+	'''Sensitivity'''
		+	*Signal to Noise: 500:1 rms, using the Raman band of water with excitation at 350 nm, exciat
	'''Physical'''<br>		'''Physical'''<br>
	*Dimensions (W×D×H): 450(W) x 490(D) x 270(H) and Weight is 15kg<br>		*Dimensions (W×D×H): 450(W) x 490(D) x 270(H) and Weight is 15kg<br>