AU Biomaterials Design Lab:Equipment/Shimadzu AA-6200

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American University
Beeghly, Room 203
4400 Massachusetts Ave NW
Washington, DC 20016

Revision as of 14:22, 28 November 2012



  • Shimadzu AA-6200 double-beam atomic absorption spectrophotometer.
  • It can detect the concentration of Au and Cu even at very low concentrations (ppm).

Purpose of Use


American University Beeghly, Room 203 4400 Massachusetts Ave NW Washington, DC 20016


  • Operation of the Shimadzu AA-6200
    1. Turn on the instrument and open the WizAArd program.
    2. Click Connect, then Next.
    3. Select the element, either Au or Cu, to be measured.
    4. Click Warmup Lamp, then Next.
    5. Adjust the lamp to the proper position by rotating it on the right side of the instrument. Click Next.
    6. Select the calibration method, enter the number of standards to be measured, and their concentrations. Click Next.
    7. Enter the number of samples to be measured and their identifications. Click Next.
    8. Ignite the flame by simultaneously pushing and holding the back and white buttons. Once the flame has ignited, release the buttons.
    9. Place the suction tube in deionized water, then click Blank to blank the instrument.
    10. Autozero by placing the suction tube in a solution of your choice, then clicking Autozero.
    11. Place the suction tube in deionized water between measurement of solutions.
    12. Place the suction tube in the first standard solution and click Start.
    13. Repeat the last two steps for all of the standard solutions, then continue on to the sample solutions.
    14. Extinguish the flame by pushing the red (extinguish) button.
    15. Click File -> Export Data to save the data as a text file.



  • Optics: Double beam, chopper mirror
  • Monochromator: Czerny-Turner monochromator, holographic grating (1600 lines/mm)
  • Wavelength range: 190-900 nm, automated wavelength selection
  • Slit width: 0.2 nm or 0.7 nm, manual selection
  • Lamp turret: 2 lamps, simultaneously lit, manual selection
  • Lamp mode: Emission, non-BGC, BGC-D2
  • Frequency: 100 Hz
  • Nebulizer: Nebulizer integrated impact bead and jacket tip, Pt/Ir capillary
  • Chamber: Polypropylene
  • Burner: Fixed position and height, 10 cm titanium slot burner, manual selection between air/C2H2 and N2O/C2H2
  • Gas control: Manual selection of flow rate, automatic air/N2O switching system
  • Safety: Gas pressure monitoring, automatic flame monitoring, safety interlock for misuse of burner, automatic flame extinguish upon power failure
  • Ignition: Manual selection
  • Software: Based on Microsoft Windows, WizAArd
  • Power requirements: AC220 V, 230 V, 50/60 Hz, 300 VA, requires transformer for AC115 V
  • Ambient temperature range: 10~35°C
  • Ambient humidity range: 45~80%, but less than 70% if temperature is above 30°C


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