AU Biomaterials Design Lab:Protocols/Autoclave - Revision history

Kathryn Muratore at 17:28, 18 August 2011

2011-08-18T17:28:32Z

←Older revision		Revision as of 17:28, 18 August 2011
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	==References==		==References==
		+
		+	[[Category:Protocol]]
		+	[[Category:AU-BDL]]

Matt Hartings at 18:04, 10 August 2011

2011-08-10T18:04:47Z

←Older revision		Revision as of 18:04, 10 August 2011
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	==Protocol==		==Protocol==
	*Make certain that there is enough water in the reservoir before you begin.		*Make certain that there is enough water in the reservoir before you begin.
-	#) Turn the power on	+	# Turn the power on
-	#) Turn the black knob to fill. This fills the chamber with water.	+	# Turn the black knob to fill. This fills the chamber with water.
-	#) Let water continue to fill the chamber until it reaches the line at the front of the chamber.	+	# Let water continue to fill the chamber until it reaches the line at the front of the chamber.
-	#) Turn the black knob to STE (short for "sterilize").	+	# Turn the black knob to STE (short for "sterilize").
-	#) Load your media into the autoclave chamber. (Note: 2 2.8L Fernbach Flasks can be loaded into the chamber)	+	# Load your media into the autoclave chamber. (Note: 2 2.8L Fernbach Flasks can be loaded into the chamber)
-	#) Close the autoclave door and seal it shut.	+	# Close the autoclave door and seal it shut.
-	#) Set the temperature knob just to the right of 121F.	+	# Set the temperature knob just to the right of 121F.
-	#) Turn the timer knob to 50 minutes.	+	# Turn the timer knob to 50 minutes.
-	#) After the 50 minutes have elapsed, wait 30 minutes until the pressure drops back to atmospheric pressure.	+	# After the 50 minutes have elapsed, wait 30 minutes until the pressure drops back to atmospheric pressure.
-	#) Turn the black knob to Off.	+	# Turn the black knob to Off.
-	#) Use gloves to unload your sterilized material.	+	# Use gloves to unload your sterilized material.

	==Notes==		==Notes==

	==References==		==References==

Matt Hartings at 18:04, 10 August 2011

2011-08-10T18:04:23Z

←Older revision		Revision as of 18:04, 10 August 2011
Line 4:		Line 4:
	==Protocol==		==Protocol==
	*Make certain that there is enough water in the reservoir before you begin.		*Make certain that there is enough water in the reservoir before you begin.
-	\|1) Turn the power on	+	#) Turn the power on
-	\|2) Turn the black knob to fill. This fills the chamber with water.	+	#) Turn the black knob to fill. This fills the chamber with water.
-	\|3) Let water continue to fill the chamber until it reaches the line at the front of the chamber.	+	#) Let water continue to fill the chamber until it reaches the line at the front of the chamber.
-	\|4) Turn the black knob to STE (short for "sterilize").	+	#) Turn the black knob to STE (short for "sterilize").
-	\|5) Load your media into the autoclave chamber. (Note: 2 2.8L Fernbach Flasks can be loaded into the chamber)	+	#) Load your media into the autoclave chamber. (Note: 2 2.8L Fernbach Flasks can be loaded into the chamber)
-	\|6) Close the autoclave door and seal it shut.	+	#) Close the autoclave door and seal it shut.
-	\|7) Set the temperature knob just to the right of 121F.	+	#) Set the temperature knob just to the right of 121F.
-	\|8) Turn the timer knob to 50 minutes.	+	#) Turn the timer knob to 50 minutes.
-	\|9) After the 50 minutes have elapsed, wait 30 minutes until the pressure drops back to atmospheric pressure.	+	#) After the 50 minutes have elapsed, wait 30 minutes until the pressure drops back to atmospheric pressure.
-	~~\|10~~) Turn the black knob to Off.	+	#) Turn the black knob to Off.
-	~~\|11~~) Use gloves to unload your sterilized material.	+	#) Use gloves to unload your sterilized material.

	==Notes==		==Notes==

	==References==		==References==

Matt Hartings at 18:01, 10 August 2011

2011-08-10T18:01:11Z

Matt Hartings at 17:59, 10 August 2011

2011-08-10T17:59:27Z

←Older revision		Revision as of 17:59, 10 August 2011
Line 1:		Line 1:
		+	==Description==
	An autoclave is a piece of equipment that is used for sterilization. In this lab, we primarily use the autoclave to prepare broth and flasks for bacterial protein expression. The autoclave that we use is located in Beeghly 314. There are directions posted on the wall in this room for proper use of the autoclave. We follow the directions for "Wet Samples".		An autoclave is a piece of equipment that is used for sterilization. In this lab, we primarily use the autoclave to prepare broth and flasks for bacterial protein expression. The autoclave that we use is located in Beeghly 314. There are directions posted on the wall in this room for proper use of the autoclave. We follow the directions for "Wet Samples".

-	Protocol:	+	==Protocol==
	*Make certain that there is enough water in the reservoir before you begin.		*Make certain that there is enough water in the reservoir before you begin.
	1) Turn the power on		1) Turn the power on
Line 14:		Line 15:
	10) Turn the black knob to Off.		10) Turn the black knob to Off.
	11) Use gloves to unload your sterilized material.		11) Use gloves to unload your sterilized material.
		+
		+	==Notes==
		+
		+	==References==

Matt Hartings: New page: An autoclave is a piece of equipment that is used for sterilization. In this lab, we primarily use the autoclave to prepare broth and flasks for bacterial protein expression. The autoclave...

2011-08-10T17:55:08Z

New page: An autoclave is a piece of equipment that is used for sterilization. In this lab, we primarily use the autoclave to prepare broth and flasks for bacterial protein expression. The autoclave...

New page

An autoclave is a piece of equipment that is used for sterilization. In this lab, we primarily use the autoclave to prepare broth and flasks for bacterial protein expression. The autoclave that we use is located in Beeghly 314. There are directions posted on the wall in this room for proper use of the autoclave. We follow the directions for "Wet Samples".

Protocol:
*Make certain that there is enough water in the reservoir before you begin.
1) Turn the power on
2) Turn the black knob to fill. This fills the chamber with water.
3) Let water continue to fill the chamber until it reaches the line at the front of the chamber.
4) Turn the black knob to STE (short for "sterilize").
5) Load your media into the autoclave chamber. (Note: 2 2.8L Fernbach Flasks can be loaded into the chamber)
6) Close the autoclave door and seal it shut.
7) Set the temperature knob just to the right of 121F.
8) Turn the timer knob to 50 minutes.
9) After the 50 minutes have elapsed, wait 30 minutes until the pressure drops back to atmospheric pressure.
10) Turn the black knob to Off.
11) Use gloves to unload your sterilized material.

←Older revision		Revision as of 18:01, 10 August 2011
Line 4:		Line 4:
	==Protocol==		==Protocol==
	*Make certain that there is enough water in the reservoir before you begin.		*Make certain that there is enough water in the reservoir before you begin.
-	1) Turn the power on	+	\|1) Turn the power on
-	2) Turn the black knob to fill. This fills the chamber with water.	+	\|2) Turn the black knob to fill. This fills the chamber with water.
-	3) Let water continue to fill the chamber until it reaches the line at the front of the chamber.	+	\|3) Let water continue to fill the chamber until it reaches the line at the front of the chamber.
-	4) Turn the black knob to STE (short for "sterilize").	+	\|4) Turn the black knob to STE (short for "sterilize").
-	5) Load your media into the autoclave chamber. (Note: 2 2.8L Fernbach Flasks can be loaded into the chamber)	+	\|5) Load your media into the autoclave chamber. (Note: 2 2.8L Fernbach Flasks can be loaded into the chamber)
-	6) Close the autoclave door and seal it shut.	+	\|6) Close the autoclave door and seal it shut.
-	7) Set the temperature knob just to the right of 121F.	+	\|7) Set the temperature knob just to the right of 121F.
-	8) Turn the timer knob to 50 minutes.	+	\|8) Turn the timer knob to 50 minutes.
-	9) After the 50 minutes have elapsed, wait 30 minutes until the pressure drops back to atmospheric pressure.	+	\|9) After the 50 minutes have elapsed, wait 30 minutes until the pressure drops back to atmospheric pressure.
-	10) Turn the black knob to Off.	+	\|10) Turn the black knob to Off.
-	11) Use gloves to unload your sterilized material.	+	\|11) Use gloves to unload your sterilized material.

	==Notes==		==Notes==

	==References==		==References==