AU Biomaterials Design Lab:Protocols/PCR Mutation Protocol: Difference between revisions
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(New page: ==Materials== * primers * plasmid *dexynucleoside triphosphate mixture (dNTPs) ==Equipment== PCR tubes temperature cycler ==Directions== *for DNA vectors (plasmids) of less than 10 kba...) |
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Revision as of 09:56, 15 October 2012
Materials
- primers
- plasmid
- dexynucleoside triphosphate mixture (dNTPs)
Equipment
PCR tubes temperature cycler
Directions
- for DNA vectors (plasmids) of less than 10 kbases)
- The primers must each be at 100-200 nh/mL
- dNTPS 100-250 μM of each dNTPs
- denaturing temperature is 95 oC
- extension temperature is 72 oC
