AU Biomaterials Design Lab:Protocols/Starter Culture Media
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(New page: ==Description== Starter cultures enable us to get a jump-start on culturing bacteria to log-phase while we are expressing proteins. A starter culture consists of a ==Protocol== ==Notes==...) |
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==Description== | ==Description== | ||
| - | Starter cultures enable us to get a jump-start on culturing bacteria to log-phase while we are expressing proteins. | + | Starter cultures enable us to get a jump-start on culturing bacteria to log-phase while we are expressing proteins. We will usually make 4 starter cultures for our expressions. |
| + | |||
==Protocol== | ==Protocol== | ||
| + | # Prepare [[AU_Biomaterials_Design_Lab:Materials/LB|LB]] in a 250mL Erlenmeyer flask | ||
| + | ## 0.875g of LB | ||
| + | ## 35mL of water | ||
| + | # Cover the flask with foil | ||
| + | # Autoclave | ||
| + | # Allow the flask to cool | ||
| + | # Add 35uL of 1000X Antibiotic | ||
| + | # Inoculate with the bacteria you are culturing using one of the two following methods | ||
| + | ## Using sterile methods, retrieve a single colony from a petrie dish and drop it into the flask | ||
| + | ## Using sterile methods, add 5uL of a glycerol stock to the flask | ||
| + | # Place the flask in a shaker/incubator and culture overnight at 37C and 200rpm | ||
==Notes== | ==Notes== | ||
==References== | ==References== | ||
Current revision
Contents |
Description
Starter cultures enable us to get a jump-start on culturing bacteria to log-phase while we are expressing proteins. We will usually make 4 starter cultures for our expressions.
Protocol
- Prepare LB in a 250mL Erlenmeyer flask
- 0.875g of LB
- 35mL of water
- Cover the flask with foil
- Autoclave
- Allow the flask to cool
- Add 35uL of 1000X Antibiotic
- Inoculate with the bacteria you are culturing using one of the two following methods
- Using sterile methods, retrieve a single colony from a petrie dish and drop it into the flask
- Using sterile methods, add 5uL of a glycerol stock to the flask
- Place the flask in a shaker/incubator and culture overnight at 37C and 200rpm
