AU Biomaterials Design Lab:Protocols/Starter Culture Media - Revision history

Matt Hartings at 22:54, 11 August 2011

Matt Hartings — Thu, 11 Aug 2011 22:54:15 GMT

←Older revision		Revision as of 22:54, 11 August 2011
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	==Description==		==Description==
-	Starter cultures enable us to get a jump-start on culturing bacteria to log-phase while we are expressing proteins.	+	Starter cultures enable us to get a jump-start on culturing bacteria to log-phase while we are expressing proteins. We will usually make 4 starter cultures for our expressions.

	==Protocol==		==Protocol==
-	# Prepare LB in a 250mL Erlenmeyer flask	+	# Prepare [[AU_Biomaterials_Design_Lab:Materials/LB\|LB]] in a 250mL Erlenmeyer flask
	## 0.875g of LB		## 0.875g of LB
	## 35mL of water		## 35mL of water

Matt Hartings: /* Protocol */

Matt Hartings — Wed, 10 Aug 2011 18:45:40 GMT

Protocol

←Older revision		Revision as of 18:45, 10 August 2011
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	==Protocol==		==Protocol==
		+	# Prepare LB in a 250mL Erlenmeyer flask
		+	## 0.875g of LB
		+	## 35mL of water
		+	# Cover the flask with foil
		+	# Autoclave
		+	# Allow the flask to cool
		+	# Add 35uL of 1000X Antibiotic
		+	# Inoculate with the bacteria you are culturing using one of the two following methods
		+	## Using sterile methods, retrieve a single colony from a petrie dish and drop it into the flask
		+	## Using sterile methods, add 5uL of a glycerol stock to the flask
		+	# Place the flask in a shaker/incubator and culture overnight at 37C and 200rpm

	==Notes==		==Notes==

	==References==		==References==

Matt Hartings: /* Description */

Matt Hartings — Wed, 10 Aug 2011 18:37:20 GMT

Description

←Older revision		Revision as of 18:37, 10 August 2011
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	==Description==		==Description==
-	Starter cultures enable us to get a jump-start on culturing bacteria to log-phase while we are expressing proteins. ~~A starter culture consists of a~~	+	Starter cultures enable us to get a jump-start on culturing bacteria to log-phase while we are expressing proteins.
		+
	==Protocol==		==Protocol==

Matt Hartings: New page: ==Description== Starter cultures enable us to get a jump-start on culturing bacteria to log-phase while we are expressing proteins. A starter culture consists of a ==Protocol== ==Notes==...

Matt Hartings — Wed, 10 Aug 2011 18:34:03 GMT

New page: ==Description== Starter cultures enable us to get a jump-start on culturing bacteria to log-phase while we are expressing proteins. A starter culture consists of a ==Protocol== ==Notes==...

New page

==Description==
Starter cultures enable us to get a jump-start on culturing bacteria to log-phase while we are expressing proteins. A starter culture consists of a
==Protocol==

==Notes==

==References==