Acinetobacter ADP1: Difference between revisions

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(New page: == Cultures == * ADP1 can be grown anywhere between room temperature and 37°C * Will not survive at 4°C * For long term storage, add 10% DMSO and store at -80°C * Naturally resistant to...)
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Revision as of 12:15, 16 June 2009

Cultures

  • ADP1 can be grown anywhere between room temperature and 37°C
  • Will not survive at 4°C
  • For long term storage, add 10% DMSO and store at -80°C
  • Naturally resistant to ampicillin and chloramphenicol. Use spectinomycin, kanamycin, or tetracycline for selections.

Transformations

Constructs

  • 2.1 in plasmid IMBB
  • 2.2 in plasmid IMBB
  • 2.1-P1010-2.2 in plasmid pSB1AC3
  • 2.1-J04450-P1010-2.2 in plasmid pSB1AC3