Affinity purification: Difference between revisions
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Before you begin, you will need at least 1mg of protein (eg.GST-FAA-N) and at least 1mg of ‘clearing’ protein (probably GST) | Before you begin, you will need at least 1mg of protein (eg.GST-FAA-N) and at least 1mg of ‘clearing’ protein (probably GST) | ||
Revision as of 12:38, 18 August 2006
Before you begin, you will need at least 1mg of protein (eg.GST-FAA-N) and at least 1mg of ‘clearing’ protein (probably GST)
This means that you will probably need 2 columns and twice as much reagent
The clearing protein will be used to make its own column in the exact same way as the fusion protein column
If you are using peptide for this column you will skip step A.
