Agarose Gel Solution: Difference between revisions
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Revision as of 14:01, 28 April 2009
- Mix 1g of gel powder with 100 mL of 1xTBE
- Microwave solution for approximately 30-60 seconds until it boils and becomes clear
- Once it is done, let it cool for about 10 minutes
- Then add ethidium bromide (2 drops, ~8ul) *EtBr is a carcinogen*
- Pour soultion into mold and use comb to brush air bubbles to the side
- Insert comb and wait for gel to set
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