Agarose Gel Solution

From OpenWetWare
Jump to navigationJump to search
  • Mix 1g of gel powder with 100 mL of 1xTBE
  • Microwave solution for approximately 30-60 seconds until it boils and becomes clear
  • Once it is done, let it cool for about 10 minutes
  • Then add ethidium bromide (2 drops, ~8ul) *EtBr is a carcinogen*
  • Pour soultion into mold and use comb to brush air bubbles to the side
  • Insert comb and wait for gel to set

<html> <a href="http://maps.google.com/maps?f=q&amp;source=embed&amp;hl=en&amp;geocode=&amp;q=420+Washington+Ave.+SE+minneapolis,+MN+55455&amp;sll=37.0625,-95.677068&amp;sspn=41.95363,67.412109&amp;ie=UTF8&amp;ll=44.982649,-93.229208&amp;spn=0.009184,0.016458&amp;z=14&amp;iwloc=A&amp;layer=c&amp;cbll=44.97366,-93.232914&amp;panoid=a2L7PqI3Y15hZyvcgEbaRQ&amp;cbp=12,147.60808515227833,,0,-16.953746390970437" style="color:#0000FF;text-align:left">View Building/Map</a></small"> </a> </html>