Agarose gel electrophoresis

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#Run the gel
#Run the gel
#Image the gel
#Image the gel
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== Buffers ==
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* [[TAE]] - better resolution of fragments >4kb;
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* [[TBE]] - better resolution of 0.1-3kb fragments; TBE is better suited for high-voltage (>150V) electrophoresis than TAE because of its higher buffering capacity and lower conductivity;
== Specific Protocols ==
== Specific Protocols ==

Revision as of 08:22, 28 June 2006

Contents

General Information

General Procedure

  1. Cast a gel
  2. Place it in gel box in running buffer
  3. Load samples
  4. Run the gel
  5. Image the gel

Buffers

  • TAE - better resolution of fragments >4kb;
  • TBE - better resolution of 0.1-3kb fragments; TBE is better suited for high-voltage (>150V) electrophoresis than TAE because of its higher buffering capacity and lower conductivity;

Specific Protocols

Endy:Agarose gel

Knight:Agarose gel electrophoresis

Notes

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