Agarose gel electrophoresis: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(table for loading dyes) |
No edit summary |
||
| Line 42: | Line 42: | ||
*If you are getting unexpected bands on your gel you may want to look at the [[Agarose gel electrophoresis/Common issues | common issues in agarose gel electrophoresis page]]. | *If you are getting unexpected bands on your gel you may want to look at the [[Agarose gel electrophoresis/Common issues | common issues in agarose gel electrophoresis page]]. | ||
*If you have no experience with gel electrophoresis or are explaining it to someone new, [http://arbl.cvmbs.colostate.edu/hbooks/genetics/biotech/gels/virgel.html here] is a cute java demo of what happens. | *If you have no experience with gel electrophoresis or are explaining it to someone new, [http://arbl.cvmbs.colostate.edu/hbooks/genetics/biotech/gels/virgel.html here] is a cute java demo of what happens. | ||
[[Category:Protocol]] | |||
Revision as of 09:26, 10 July 2006
General Information
General Procedure
- Cast a gel
- Place it in gel box in running buffer
- Load samples
- Run the gel
- Image the gel
Buffers
- TAE - better resolution of fragments >4kb;
- TBE - better resolution of 0.1-3kb fragments; TBE is better suited for high-voltage (>150V) electrophoresis than TAE because of its higher buffering capacity and lower conductivity;
Loading dyes
| dye | 0.5-1.5% agarose | 2.0-3.0% agarose* |
| xylene cyanol | 4000-5000 bp | 750 bp |
| bromophenol blue | 400-500 bp | 100 bp |
*sieving agarose
Specific Protocols
Knight:Agarose gel electrophoresis
Notes
- If you are getting unexpected bands on your gel you may want to look at the common issues in agarose gel electrophoresis page.
- If you have no experience with gel electrophoresis or are explaining it to someone new, here is a cute java demo of what happens.
