Agarose gel electrophoresis: Difference between revisions
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#Run the gel | #Run the gel | ||
#Image the gel | #Image the gel | ||
== Buffers == | |||
* [[TAE]] - better resolution of fragments >4kb; | |||
* [[TBE]] - better resolution of 0.1-3kb fragments; TBE is better suited for high-voltage (>150V) electrophoresis than TAE because of its higher buffering capacity and lower conductivity; | |||
== Specific Protocols == | == Specific Protocols == | ||
Revision as of 05:22, 28 June 2006
General Information
General Procedure
- Cast a gel
- Place it in gel box in running buffer
- Load samples
- Run the gel
- Image the gel
Buffers
- TAE - better resolution of fragments >4kb;
- TBE - better resolution of 0.1-3kb fragments; TBE is better suited for high-voltage (>150V) electrophoresis than TAE because of its higher buffering capacity and lower conductivity;
Specific Protocols
Knight:Agarose gel electrophoresis
Notes
- If you are getting unexpected bands on your gel you may want to look at the common issues in agarose gel electrophoresis page.
- If you have no experience with gel electrophoresis or are explaining it to someone new, here is a cute java demo of what happens.
